High mobility group B1 protein suppresses the human plasmacytoid dendritic cell response to TLR9 agonists

Petar J. Popovic, Richard DeMarco, Michael T. Lotze, Steven E. Winikoff, David L. Bartlett, Arthur M. Krieg, Z. Sheng Guo, Charles K. Brown, Kevin J. Tracey, Herbert J. Zeh

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Plasmacytoid dendritic cells (PDC) are innate immune effector cells that are recruited to sites of chronic inflammation, where they modify the quality and nature of the adaptive immune response. PDCs modulate adaptive immunity in response to signals delivered within the local inflammatory milieu by pathogen- or damage-associated molecular pattern, molecules, and activated immune cells (including NK, T, and myeloid dendritic cells). High mobility group B1 (HMGB1) is a recently identified damage-associated molecular pattern that is released during necrotic cell death and also secreted from activated macrophages, NK cells, and mature myeloid dendritic cells. We have investigated the effect of HMGB1 on the function of PDCs. In this study, we demonstrate that HMGB1 suppresses PDC cytokine secretion and maturation in response to TLR9 agonists including the hypomethylated oligodeoxynucleotide CpG- and DNA-containing viruses. HMGB1-inhibited secretion of several proinflammatory cytokines including IFN-α, IL-6, TNF-α, inducible protein-10, and IL-12. In addition, HMGB1 prevented the CpG induced up-regulation of costimulatory molecules on the surface of PDC and potently suppressed their ability to drive generation of IFN-γ-secreting T cells. Our observations suggest that HMGB1 may play a critical role in regulating the immune response during chronic inflammation and tissue damage through modulation of PDC function.

Original languageEnglish (US)
Pages (from-to)8701-8707
Number of pages7
JournalJournal of Immunology
Volume177
Issue number12
DOIs
StatePublished - Dec 15 2006
Externally publishedYes

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High Mobility Group Proteins
Dendritic Cells
Adaptive Immunity
Myeloid Cells
Natural Killer Cells
Cytokines
Inflammation
DNA Viruses
Oligodeoxyribonucleotides
Interleukin-12
Interleukin-6
Cell Death
Up-Regulation
Macrophages
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

High mobility group B1 protein suppresses the human plasmacytoid dendritic cell response to TLR9 agonists. / Popovic, Petar J.; DeMarco, Richard; Lotze, Michael T.; Winikoff, Steven E.; Bartlett, David L.; Krieg, Arthur M.; Guo, Z. Sheng; Brown, Charles K.; Tracey, Kevin J.; Zeh, Herbert J.

In: Journal of Immunology, Vol. 177, No. 12, 15.12.2006, p. 8701-8707.

Research output: Contribution to journalArticle

Popovic, PJ, DeMarco, R, Lotze, MT, Winikoff, SE, Bartlett, DL, Krieg, AM, Guo, ZS, Brown, CK, Tracey, KJ & Zeh, HJ 2006, 'High mobility group B1 protein suppresses the human plasmacytoid dendritic cell response to TLR9 agonists', Journal of Immunology, vol. 177, no. 12, pp. 8701-8707. https://doi.org/10.4049/jimmunol.177.12.8701
Popovic, Petar J. ; DeMarco, Richard ; Lotze, Michael T. ; Winikoff, Steven E. ; Bartlett, David L. ; Krieg, Arthur M. ; Guo, Z. Sheng ; Brown, Charles K. ; Tracey, Kevin J. ; Zeh, Herbert J. / High mobility group B1 protein suppresses the human plasmacytoid dendritic cell response to TLR9 agonists. In: Journal of Immunology. 2006 ; Vol. 177, No. 12. pp. 8701-8707.
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