High-mobility group box 1 suppresses resolvin D1-induced phagocytosis via induction of resolvin D1-inactivating enzyme, 15-hydroxyprostaglandin dehydrogenase

Gyeoung Jin Kang, Hye Ja Lee, Yun Pyo Kang, Eun Ji Kim, Hyun Ji Kim, Hyun Jung Byun, Mi Kyung Park, Hoon Cho, Sung Won Kwon, Chang Hoon Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

High-mobility group box 1 (HMGB1) enhances inflammatory reactions by potentiating the activity of pro-inflammatory mediators and suppressing the phagocytosis of apoptotic neutrophils. However, the effects of HMGB1 on phagocytosis induced by pro-resolving mediators, such as resolvins, have not been studied up until this point. In this study, we investigated the effects and underlying mechanism of HMGB1 on resolvin D1-induced phagocytosis of MDA-MB-231 cells, which were selected as a model system based on their phagocytic capability and ease of transfecting them with a plasmid or siRNA in several cancer cell lines. Then we confirmed effects of HMGB1 in THP-1 cells.Resolvin D1 (RvD1) enhanced phagocytosis in MDA-MB-231 and THP-1 cells. HMGB1 suppressed RvD1-induced phagocytosis in MDA-MB.231 and THP-1 cells. HMGB1 dose-dependently induced the expression of 15-hydroxyprostaglandin dehydrogenase (15-PGDH), the inactivating enzyme in pro-resolving lipid mediators such as RvE1 and RvD1. Involvement of 15-PGDH in-HMGB-1-induced suppression of phagocytosis was examined using siRNA of 15-PGDH or 15-PGDH inhibitor, TD23. Surprisingly, the silencing of 15-PGDH increased phagocytotic activity of MDA-MB-231 cells. TD23 also enhanced phagocytosis of MDA-MB-231 and THP-1 cells.In conclusion, the release of HMGB1 during the inflammatory phase induces 15-PGDH expression, which suppresses the phagocytotic activity of macrophages. These processes might be involved in the mechanism that blocks the resolution of inflammation, thereby allowing acute inflammation to progress to chronic inflammation.

Original languageEnglish (US)
Pages (from-to)1981-1988
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1852
Issue number9
DOIs
StatePublished - Sep 1 2015

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15-hydroxyprostaglandin dehydrogenase
Phagocytosis
Enzymes
Inflammation
Small Interfering RNA
HMGB Proteins
resolvin D1
Neutrophils
Plasmids

Keywords

  • 15-PGDH
  • HMGB1
  • Phagocytosis
  • Resolution of inflammation
  • Resolvin D1

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine

Cite this

High-mobility group box 1 suppresses resolvin D1-induced phagocytosis via induction of resolvin D1-inactivating enzyme, 15-hydroxyprostaglandin dehydrogenase. / Kang, Gyeoung Jin; Lee, Hye Ja; Kang, Yun Pyo; Kim, Eun Ji; Kim, Hyun Ji; Byun, Hyun Jung; Park, Mi Kyung; Cho, Hoon; Kwon, Sung Won; Lee, Chang Hoon.

In: Biochimica et Biophysica Acta - Molecular Basis of Disease, Vol. 1852, No. 9, 01.09.2015, p. 1981-1988.

Research output: Contribution to journalArticle

Kang, Gyeoung Jin ; Lee, Hye Ja ; Kang, Yun Pyo ; Kim, Eun Ji ; Kim, Hyun Ji ; Byun, Hyun Jung ; Park, Mi Kyung ; Cho, Hoon ; Kwon, Sung Won ; Lee, Chang Hoon. / High-mobility group box 1 suppresses resolvin D1-induced phagocytosis via induction of resolvin D1-inactivating enzyme, 15-hydroxyprostaglandin dehydrogenase. In: Biochimica et Biophysica Acta - Molecular Basis of Disease. 2015 ; Vol. 1852, No. 9. pp. 1981-1988.
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AU - Kim, Hyun Ji

AU - Byun, Hyun Jung

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