High-order epistasis in catalytic power of dihydrofolate reductase gives rise to a rugged fitness landscape in the presence of trimethoprim selection

Earth & Environmental Sciences

• epistasis 100%
• mutation 64%
• fitness 63%
• mutant 45%
• enzyme 34%
• in vitro assay 32%
• antibiotics 21%
• targeting 21%
• inhibitor 20%
• library 20%
• experimental study 17%
• protein 15%
• parameter 14%
• substrate 14%
• kinetics 13%
• effect 5%

Agriculture & Biology

• dihydrofolate reductase 85%
• epistasis 77%
• trimethoprim 75%
• mutation 22%
• mutants 12%
• catalytic activity 10%
• enzymes 9%
• antibiotics 8%
• kinetics 7%
• Escherichia coli 6%
• assays 5%
• proteins 3%

Medicine & Life Sciences

• Tetrahydrofolate Dehydrogenase 86%
• Trimethoprim 83%
• Mutation 18%
• Enzymes 11%
• Kinetics 7%
• Escherichia coli 7%
• Anti-Bacterial Agents 7%
• In Vitro Techniques 4%
• Proteins 3%