High-performance liquid chromatographic separation of cobalamins

Eugene P. Frenkel, Richard L. Kitchens, Russell Prough

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Physiological cobalamins were separated by means of high-performance liquid chromatography (HPLC). Optimal conditions for elution of methylcobalamin, adenosylcobalamin, hydroxycobalamin and cyanocobalamin were determined. Excellent separation and resolution of these physiological cobalamins by HPLC were achieved. In addition, several cobalamin analogues were also studied and shown to be separable from the physiological forms. HPLC provides a rapid, sensitive, reproducible means of characterizing physiological cobalamins.

Original languageEnglish (US)
Pages (from-to)393-400
Number of pages8
JournalJournal of Chromatography A
Volume174
Issue number2
DOIs
StatePublished - Jul 1 1979

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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