TY - JOUR
T1 - High-performance liquid chromatographic separation of cobalamins
AU - Frenkel, Eugene P.
AU - Kitchens, Richard L.
AU - Prough, Russell
N1 - Funding Information:
This work was supported by NIH Grant No. l-POl-CA23115-01; Veterans Administration EIospital MRIS 1450; the Heddens-Good Foundation; the McDer-mott Foundation, and the Robert A. Welch Foundation (Grant I-616). Dr. Prough was a USPHS Research Career Development Awardee HLCA 00255.
PY - 1979/7/1
Y1 - 1979/7/1
N2 - Physiological cobalamins were separated by means of high-performance liquid chromatography (HPLC). Optimal conditions for elution of methylcobalamin, adenosylcobalamin, hydroxycobalamin and cyanocobalamin were determined. Excellent separation and resolution of these physiological cobalamins by HPLC were achieved. In addition, several cobalamin analogues were also studied and shown to be separable from the physiological forms. HPLC provides a rapid, sensitive, reproducible means of characterizing physiological cobalamins.
AB - Physiological cobalamins were separated by means of high-performance liquid chromatography (HPLC). Optimal conditions for elution of methylcobalamin, adenosylcobalamin, hydroxycobalamin and cyanocobalamin were determined. Excellent separation and resolution of these physiological cobalamins by HPLC were achieved. In addition, several cobalamin analogues were also studied and shown to be separable from the physiological forms. HPLC provides a rapid, sensitive, reproducible means of characterizing physiological cobalamins.
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U2 - 10.1016/S0021-9673(00)86013-6
DO - 10.1016/S0021-9673(00)86013-6
M3 - Article
C2 - 546897
AN - SCOPUS:0018750014
SN - 0021-9673
VL - 174
SP - 393
EP - 400
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 2
ER -