High pressure liquid chromatographic assay of cefamandole in serum following intravenous and intraperitoneal administration

R. E. Bawdon, K. J. Leveno, J. G. Quirk

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Following cesarean section 102 women were treated with cefamandole by either perioperative intravenous administration or intraperitoneal irrigation. High-pressure liquid chromatographic (HPLC) methods for the quantitation of the low serum levels of cefamandole following intraperitoneal lavage were developed. The antibiotic was assayed in the serum using a standard microbiological assay and two types of reverse phase column technology for HPLC. The two HPLC systems were almost identical in performance. Both HPLC methods were at least 10-fold more sensitive than the microbiological assay. The correlation between the three methods was 0.9739. The half-life of cefamandole was 37 min, which was not significantly different from the half-life of the drug in serum of non-pregnant women. The peak serum levels were 47.6 ± 36.8 μg/ml and 1.98 ± 1.5 μg/ml for the intravenous and intraperitoneal methods of administration, respectively.

Original languageEnglish (US)
Pages (from-to)2747-2759
Number of pages13
JournalJournal of Liquid Chromatography
Issue number14
StatePublished - Dec 1 1983


ASJC Scopus subject areas

  • Molecular Medicine

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