An analytic high-pressure liquid chromatographic (HPLC) procedure for the assay of desacetylcefotaxime and cefotaxime in gynecologic tissue was developed. Normal individuals undergoing elective hysterectomy were subjects in this study. Blood and myometrium were removed up to four hours after a 1-g intramuscular dose of cefotaxime. Since cefotaxime is unstable in homogenized tissue at room temperature, the specimens must be maintained at 4°C during homogenization and extraction. Mean serum desacetyl-cefotaxime and cefotaxime levels were 3.2 ± 2.0 qg/ml and 6.8 ± 4.4 pg/ml, respectively. The mean myometrium concentrations of desacetylcefotaxime and cefotaxime were 8.4 ± 10.0 pg/g and 6.3 ± 8.9 pg/g, respectively. The cefotaxime to desacetylcefotaxime ratios in serum and tissue were 2.12 and 0.75, respectively. Our results suggest that in antimicrobial synergistic studies evaluating serum and tissue levels, the optimal ratio of one part cefotaxime to at least one part desacetylcefotaxime.
ASJC Scopus subject areas
- Molecular Medicine