High-pressure liquid chromatographic assay of cefotaxime and desacetylcefotaxime in human myometrium

R. E. Bawdon, W. J. Novick, D. L. Hemsell, W. D. Welch

Research output: Contribution to journalArticle

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Abstract

An analytic high-pressure liquid chromatographic (HPLC) procedure for the assay of desacetylcefotaxime and cefotaxime in gynecologic tissue was developed. Normal individuals undergoing elective hysterectomy were subjects in this study. Blood and myometrium were removed up to four hours after a l-g intramuscular dose of cefotaxime. Since cefotaxime is unstable in homogenized tissue at room temperature, the specimens must be maintained at 4 degree C during homogenization and extraction. Mean serum desacetylcefotaxime and cefotaxime levels were 3. 2 plus or minus 2. 0 mu g/ml and 6. 8 plus or minus 4. 4 mu g/ml, respectively. The mean myometrium concentrations of desacetylcefotaxime and cefotaxime were 8. 4 plus or minus 10. 0 mu g/g and 6. 3 plus or minus 8. 9 mu g/g, respectively.

Original languageEnglish (US)
Pages (from-to)2483-2491
Number of pages9
JournalJournal of Liquid Chromatography
Volume7
Issue number12
StatePublished - 1984

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Cefotaxime
Myometrium
Assays
Pressure
Liquids
Tissue
Hysterectomy
Blood
desacetylcefotaxime
Temperature
Serum

ASJC Scopus subject areas

  • Molecular Medicine

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High-pressure liquid chromatographic assay of cefotaxime and desacetylcefotaxime in human myometrium. / Bawdon, R. E.; Novick, W. J.; Hemsell, D. L.; Welch, W. D.

In: Journal of Liquid Chromatography, Vol. 7, No. 12, 1984, p. 2483-2491.

Research output: Contribution to journalArticle

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