High refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells

Edgar Gutierrez; Eugene Tkachenko; Achim Besser; Prithu Sundd; Klaus Ley; Gaudenz Danuser; Mark H. Ginsberg; Alex Groisman

doi:10.1371/journal.pone.0023807

High refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells

Edgar Gutierrez, Eugene Tkachenko, Achim Besser, Prithu Sundd, Klaus Ley, Gaudenz Danuser, Mark H. Ginsberg, Alex Groisman

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

Substrate rigidity profoundly impacts cellular behaviors such as migration, gene expression, and cell fate. Total Internal Reflection Fluorescence (TIRF) microscopy enables selective visualization of the dynamics of substrate adhesions, vesicle trafficking, and biochemical signaling at the cell-substrate interface. Here we apply high-refractive-index silicone gels to perform TIRF microscopy on substrates with a wide range of physiological elastic moduli and simultaneously measure traction forces exerted by cells on the substrate.

Original language	English (US)
Article number	e23807
Journal	PloS one
Volume	6
Issue number	9
DOIs	https://doi.org/10.1371/journal.pone.0023807
State	Published - Sep 22 2011

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences

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10.1371/journal.pone.0023807

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abstract = "Substrate rigidity profoundly impacts cellular behaviors such as migration, gene expression, and cell fate. Total Internal Reflection Fluorescence (TIRF) microscopy enables selective visualization of the dynamics of substrate adhesions, vesicle trafficking, and biochemical signaling at the cell-substrate interface. Here we apply high-refractive-index silicone gels to perform TIRF microscopy on substrates with a wide range of physiological elastic moduli and simultaneously measure traction forces exerted by cells on the substrate.",

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AU - Gutierrez, Edgar

AU - Tkachenko, Eugene

AU - Besser, Achim

AU - Sundd, Prithu

AU - Ley, Klaus

AU - Danuser, Gaudenz

AU - Ginsberg, Mark H.

AU - Groisman, Alex

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High refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells

Abstract

ASJC Scopus subject areas

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