High refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells

Edgar Gutierrez, Eugene Tkachenko, Achim Besser, Prithu Sundd, Klaus Ley, Gaudenz Danuser, Mark H. Ginsberg, Alex Groisman

Research output: Contribution to journalArticle

25 Scopus citations


Substrate rigidity profoundly impacts cellular behaviors such as migration, gene expression, and cell fate. Total Internal Reflection Fluorescence (TIRF) microscopy enables selective visualization of the dynamics of substrate adhesions, vesicle trafficking, and biochemical signaling at the cell-substrate interface. Here we apply high-refractive-index silicone gels to perform TIRF microscopy on substrates with a wide range of physiological elastic moduli and simultaneously measure traction forces exerted by cells on the substrate.

Original languageEnglish (US)
Article numbere23807
JournalPLoS One
Issue number9
StatePublished - Sep 22 2011


ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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