High-resolution analysis of ethanol-induced locomotor stimulation in Drosophila

Fred W. Wolf, Aylin R. Rodan, Linus T Y Tsai, Ulrike Heberlein

Research output: Contribution to journalArticlepeer-review

129 Scopus citations

Abstract

Understanding how ethanol influences behavior is key to deciphering the mechanisms of ethanol action and alcoholism. In mammals, low doses of ethanol stimulate locomotion, whereas high doses depress it. The acute stimulant effect of ethanol has been proposed to be a manifestation of its rewarding effects. In Drosophila, ethanol exposure transiently potentiates locomotor activity in a biphasic dose- and time-dependent manner. An initial short-lived peak of activity corresponds to an olfactory response to ethanol. A second, longer-lasting period of increased activity coincides with rising internal ethanol concentrations; these closely parallel concentrations that stimulate locomotion in mammals. High-resolution analysis of the walking pattern of individual flies revealed that locomotion consists of bouts of activity; bout structure can be quantified by bout frequency, bout length, and the time spent walking at high speeds. Ethanol exposure induces both dramatic and dynamic changes in bout structure. Mutants with increased ethanol sensitivity show distinct changes in ethanol-induced locomotor behavior, as well as genotype-specific changes in activity bout structure. Thus, the overall effect of ethanol on locomotor behavior in Drosophila is caused by changes in discrete quantifiable parameters of walking pattern. The effects of ethanol on locomotion are comparable in flies and mammals, suggesting that Drosophila is a suitable model system to study the underlying mechanisms.

Original languageEnglish (US)
Pages (from-to)11035-11044
Number of pages10
JournalJournal of Neuroscience
Volume22
Issue number24
DOIs
StatePublished - Dec 15 2002

Keywords

  • Amnesiac
  • Behavior
  • Drosophila
  • Ethanol
  • Locomotion
  • Rutabaga

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'High-resolution analysis of ethanol-induced locomotor stimulation in Drosophila'. Together they form a unique fingerprint.

Cite this