High-resolution computed tomography of single breast cancer microcalcifications in vivo

Kazumasa Inoue, Fangbing Liu, Jack Hoppin, Elaine P. Lunsford, Christian Lackas, Jacob Hesterman, Robert E. Lenkinski, Hirofumi Fujii, John V. Frangioni

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Microcalcification is a hallmark of breast cancer and a key diagnostic feature for mammography. We recently described the first robust animal model of breast cancer microcalcification. In this study, we hypothesized that high-resolution computed tomography (CT) could potentially detect the genesis of a single microcalcification in vivo and quantify its growth over time. Using a commercial CT scanner, we systematically optimized acquisition and reconstruction parameters. Two ray-tracing image reconstruction algorithms were tested: a voxel-driven "fast" cone beam algorithm (FCBA) and a detector-driven "exact" cone beam algorithm (ECBA). By optimizing acquisition and reconstruction parameters, we were able to achieve a resolution of 104 μm full width at halfmaximum (FWHM). At an optimal detector sampling frequency, the ECBA provided a 28 μm (21%) FWHM improvement in resolution over the FCBA. In vitro, we were able to image a single 300 μm Times; 100 μm hydroxyapatite crystal. In a syngeneic rat model of breast cancer, we were able to detect the genesis of a single microcalcification in vivo and follow its growth longitudinally over weeks. Taken together, this study provides an in vivo "gold standard" for the development of calcification-specific contrast agents and a model system for studying the mechanism of breast cancer microcalcification.

Original languageEnglish (US)
Pages (from-to)295-304
Number of pages10
JournalMolecular Imaging
Volume10
Issue number4
DOIs
StatePublished - Jul 2011

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics

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  • Cite this

    Inoue, K., Liu, F., Hoppin, J., Lunsford, E. P., Lackas, C., Hesterman, J., Lenkinski, R. E., Fujii, H., & Frangioni, J. V. (2011). High-resolution computed tomography of single breast cancer microcalcifications in vivo. Molecular Imaging, 10(4), 295-304. https://doi.org/10.2310/7290.2010.00050