High resolution MICA genotyping by sequence-based typing (SBT)

Yizhou Zou, Peter Stastny

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

We have developed a MICA typing method based on polymerase chain reaction (PCR) sequence-based typing and a computer program that determines the polymorphisms and distinguishes the GCT repeats in exon 5. One PCR amplification was performed to obtain templates of 2.2 kb, including exons 2, 3, 4, and 5 of MICA to be sequenced with two forward and two reverse primers. Overlay of nucleotide sequencing signals resulting from presence of different GCT repeats in exon 5 from two different MICA alleles can be identified by a computer program that analyses the combined signal string containing the 35 bases.

Original languageEnglish (US)
Title of host publicationImmunogenetics
Subtitle of host publicationMethods and Applications in Clinical Practice
EditorsFrank T. Christiansen, Brian D Tait
Pages183-195
Number of pages13
DOIs
StatePublished - Dec 1 2012

Publication series

NameMethods in Molecular Biology
Volume882
ISSN (Print)1064-3745

Keywords

  • Computer analysis
  • External domains
  • MICA typing
  • SBT
  • Transmembrane region

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Zou, Y., & Stastny, P. (2012). High resolution MICA genotyping by sequence-based typing (SBT). In F. T. Christiansen, & B. D. Tait (Eds.), Immunogenetics: Methods and Applications in Clinical Practice (pp. 183-195). (Methods in Molecular Biology; Vol. 882). https://doi.org/10.1007/978-1-61779-842-9_11