High-resolution 1H NMR spectroscopy following experimental brain trauma

Yoram Rubin, Kim Cecil, Suzanne Wehrli, Tracy K. McIntosh, Robert E. Lenkinski, Douglas H. Smith

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


We investigated acute metabolic changes following parasagittal fluid- percussion brain injury in the rat, using high-resolution 1H nuclear magnetic resonance (NMR) spectroscopy. Sixty minutes following brain injury or sham (surgery, no injury) treatment, brains were rapidly removed and the injured and control cortices were isolated (n = 5/group). Isolates of brain cortices were then placed in buffer and studied in a 400-MHz spectrometer with measurements taken every 15 min over a 145-min period. At the initial NMR evaluation (immediately following dissection), we observed significantly lower levels of N-acetyl aspartic acid (NAA) in the injured group compared to the sham group. Surprisingly, a reciprocal increase in the concentration of acetate, a major metabolic product of NAA, was not observed at this timepoint. At subsequent timepoints, a progressive loss of NAA was observed in both injured and sham cortices, presumably due to ischemic conditions of the ex vivo samples. However, this progressive loss of NAA was now accompanied by a commensurate accumulation of acetate. These results suggest that (1) a decrease in the concentration of NAA occurs by 1 h following experimental brain trauma, potentially marking traumatic neural injury; (2) the initial absence of an expected reciprocal increase in acetate concentration may signify rapid utilization of acetate following trauma, potentially for reparative processes; and (3) in contrast to trauma alone, post morterm ischemic conditions may induce an increase in acetate concentrations.

Original languageEnglish (US)
Pages (from-to)441-449
Number of pages9
JournalJournal of neurotrauma
Issue number7
StatePublished - Jul 1997


  • Acetate
  • Brain injury
  • Fluid percussion
  • N-acetyl aspartate
  • Nuclear magnetic resonance

ASJC Scopus subject areas

  • Clinical Neurology


Dive into the research topics of 'High-resolution 1H NMR spectroscopy following experimental brain trauma'. Together they form a unique fingerprint.

Cite this