High-risk corneal allografts are capable of stimulating complement dependent cytolytic antibodies

Sylvia L. Hargrave, Sherine Taherzadeh, Sushma Hegde, Jerry Niederkorn

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Purpose. To prospectively characterize serum antibody responses in high- risk corneal allografts. Methods. Orthotopic and heterotopic C3H (H-2(k)) corneas were transplanted to CB6F1 (H-2(b/d)) recipient mice. Individual serum samples were collected at days 7, 14, 21, and 28 from orthotopically and heterotopically grafted mice. Samples were tested for anti-C3H antibodies using an enzyme-linked immunosorbent assay (ELISA). Serum samples were also tested for the ability to lyse target cells in the presence of complement. Results. In high-risk donor-host combinations, 100% corneal allograft rejection occurs in the absence of IgG1 production. Both orthotopic and heterotopic corneal allografts induced cytolytic complement fixing antibody isotypes. Conclusions. Allospecific corneal antibody may be involved in corneal graft rejection in high-risk donor-host combinations.

Original languageEnglish (US)
Pages (from-to)521-525
Number of pages5
JournalCornea
Volume19
Issue number4
DOIs
StatePublished - Jul 2000

Fingerprint

Allografts
Antibodies
Serum
Graft Rejection
Cornea
Antibody Formation
Anti-Idiotypic Antibodies
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay

Keywords

  • Antibody
  • Corneal graft rejection
  • Corneal transplantation

ASJC Scopus subject areas

  • Ophthalmology

Cite this

High-risk corneal allografts are capable of stimulating complement dependent cytolytic antibodies. / Hargrave, Sylvia L.; Taherzadeh, Sherine; Hegde, Sushma; Niederkorn, Jerry.

In: Cornea, Vol. 19, No. 4, 07.2000, p. 521-525.

Research output: Contribution to journalArticle

Hargrave, Sylvia L. ; Taherzadeh, Sherine ; Hegde, Sushma ; Niederkorn, Jerry. / High-risk corneal allografts are capable of stimulating complement dependent cytolytic antibodies. In: Cornea. 2000 ; Vol. 19, No. 4. pp. 521-525.
@article{3cd77ccb1b9548d993495b18906acccf,
title = "High-risk corneal allografts are capable of stimulating complement dependent cytolytic antibodies",
abstract = "Purpose. To prospectively characterize serum antibody responses in high- risk corneal allografts. Methods. Orthotopic and heterotopic C3H (H-2(k)) corneas were transplanted to CB6F1 (H-2(b/d)) recipient mice. Individual serum samples were collected at days 7, 14, 21, and 28 from orthotopically and heterotopically grafted mice. Samples were tested for anti-C3H antibodies using an enzyme-linked immunosorbent assay (ELISA). Serum samples were also tested for the ability to lyse target cells in the presence of complement. Results. In high-risk donor-host combinations, 100{\%} corneal allograft rejection occurs in the absence of IgG1 production. Both orthotopic and heterotopic corneal allografts induced cytolytic complement fixing antibody isotypes. Conclusions. Allospecific corneal antibody may be involved in corneal graft rejection in high-risk donor-host combinations.",
keywords = "Antibody, Corneal graft rejection, Corneal transplantation",
author = "Hargrave, {Sylvia L.} and Sherine Taherzadeh and Sushma Hegde and Jerry Niederkorn",
year = "2000",
month = "7",
doi = "10.1097/00003226-200007000-00024",
language = "English (US)",
volume = "19",
pages = "521--525",
journal = "Cornea",
issn = "0277-3740",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - High-risk corneal allografts are capable of stimulating complement dependent cytolytic antibodies

AU - Hargrave, Sylvia L.

AU - Taherzadeh, Sherine

AU - Hegde, Sushma

AU - Niederkorn, Jerry

PY - 2000/7

Y1 - 2000/7

N2 - Purpose. To prospectively characterize serum antibody responses in high- risk corneal allografts. Methods. Orthotopic and heterotopic C3H (H-2(k)) corneas were transplanted to CB6F1 (H-2(b/d)) recipient mice. Individual serum samples were collected at days 7, 14, 21, and 28 from orthotopically and heterotopically grafted mice. Samples were tested for anti-C3H antibodies using an enzyme-linked immunosorbent assay (ELISA). Serum samples were also tested for the ability to lyse target cells in the presence of complement. Results. In high-risk donor-host combinations, 100% corneal allograft rejection occurs in the absence of IgG1 production. Both orthotopic and heterotopic corneal allografts induced cytolytic complement fixing antibody isotypes. Conclusions. Allospecific corneal antibody may be involved in corneal graft rejection in high-risk donor-host combinations.

AB - Purpose. To prospectively characterize serum antibody responses in high- risk corneal allografts. Methods. Orthotopic and heterotopic C3H (H-2(k)) corneas were transplanted to CB6F1 (H-2(b/d)) recipient mice. Individual serum samples were collected at days 7, 14, 21, and 28 from orthotopically and heterotopically grafted mice. Samples were tested for anti-C3H antibodies using an enzyme-linked immunosorbent assay (ELISA). Serum samples were also tested for the ability to lyse target cells in the presence of complement. Results. In high-risk donor-host combinations, 100% corneal allograft rejection occurs in the absence of IgG1 production. Both orthotopic and heterotopic corneal allografts induced cytolytic complement fixing antibody isotypes. Conclusions. Allospecific corneal antibody may be involved in corneal graft rejection in high-risk donor-host combinations.

KW - Antibody

KW - Corneal graft rejection

KW - Corneal transplantation

UR - http://www.scopus.com/inward/record.url?scp=0033926679&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033926679&partnerID=8YFLogxK

U2 - 10.1097/00003226-200007000-00024

DO - 10.1097/00003226-200007000-00024

M3 - Article

C2 - 10928771

AN - SCOPUS:0033926679

VL - 19

SP - 521

EP - 525

JO - Cornea

JF - Cornea

SN - 0277-3740

IS - 4

ER -