High-speed multiparameter photophysical analyses of fluorophore libraries

Kevin M. Dean, Lloyd M. Davis, Jennifer L. Lubbeck, Premashis Manna, Pia Friis, Amy E. Palmer, Ralph Jimenez

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


There is a critical need for high-speed multiparameter photophysical measurements of large libraries of fluorescent probe variants for imaging and biosensor development. We present a microfluidic flow cytometer that rapidly assays 104-105 member cell-based fluorophore libraries, simultaneously measuring fluorescence lifetime and photobleaching. Together, these photophysical characteristics determine imaging performance. We demonstrate the ability to resolve the diverse photophysical characteristics of different library types and the ability to identify rare populations.

Original languageEnglish (US)
Pages (from-to)5026-5030
Number of pages5
JournalAnalytical Chemistry
Issue number10
StatePublished - May 19 2015
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry


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