High-speed multiparameter photophysical analyses of fluorophore libraries

Kevin M. Dean, Lloyd M. Davis, Jennifer L. Lubbeck, Premashis Manna, Pia Friis, Amy E. Palmer, Ralph Jimenez

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

There is a critical need for high-speed multiparameter photophysical measurements of large libraries of fluorescent probe variants for imaging and biosensor development. We present a microfluidic flow cytometer that rapidly assays 104-105 member cell-based fluorophore libraries, simultaneously measuring fluorescence lifetime and photobleaching. Together, these photophysical characteristics determine imaging performance. We demonstrate the ability to resolve the diverse photophysical characteristics of different library types and the ability to identify rare populations.

Original languageEnglish (US)
Pages (from-to)5026-5030
Number of pages5
JournalAnalytical chemistry
Volume87
Issue number10
DOIs
Publication statusPublished - May 19 2015
Externally publishedYes

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ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Dean, K. M., Davis, L. M., Lubbeck, J. L., Manna, P., Friis, P., Palmer, A. E., & Jimenez, R. (2015). High-speed multiparameter photophysical analyses of fluorophore libraries. Analytical chemistry, 87(10), 5026-5030. https://doi.org/10.1021/acs.analchem.5b00607