High threshold, proximal initiation, and slow conduction velocity of action potentials in dentate granule neuron mossy fibers

Geraldine J. Kress, Margaret J. Dowling, Julian P. Meeks, Steven Mennerick

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Dentate granule neurons give rise to some of the smallest unmyelinated fibers in the mammalian CNS, the hippocampal mossy fibers. These neurons are also key regulators of physiological and pathophysiological information flow through the hippocampus. We took a comparative approach to studying mossy fiber action potential initiation and propagation in hippocampal slices from juvenile rats. Dentate granule neurons exhibited axonal action potential initiation significantly more proximal than CA3 pyramidal neurons. This conclusion was suggested by phase plot analysis of somatic action potentials and by local tetrodotoxin application to the axon and somatodendritic compartments. This conclusion was also verified by immunostaining for voltage-gated sodium channel alpha subunits and by direct dual soma/axonal recordings. Dentate neurons exhibited a significantly higher action potential threshold and slower axonal conduction velocity than CA3 neurons. We conclude that while the electrotonically proximal axon location of action potential initiation allows granule neurons to sensitively detect and integrate synaptic inputs, the neurons are sluggish to initiate and propagate an action potential.

Original languageEnglish (US)
Pages (from-to)281-291
Number of pages11
JournalJournal of Neurophysiology
Volume100
Issue number1
DOIs
StatePublished - Jul 2008

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Action Potentials
Neurons
Axons
Hippocampal Mossy Fibers
Voltage-Gated Sodium Channels
Pyramidal Cells
Tetrodotoxin
Carisoprodol
Hippocampus

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

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High threshold, proximal initiation, and slow conduction velocity of action potentials in dentate granule neuron mossy fibers. / Kress, Geraldine J.; Dowling, Margaret J.; Meeks, Julian P.; Mennerick, Steven.

In: Journal of Neurophysiology, Vol. 100, No. 1, 07.2008, p. 281-291.

Research output: Contribution to journalArticle

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