High TLR7 expression drives the expansion of CD19+CD24HiCD38hi transitional B cells and autoantibody production in SLE patients

Ting Wang, John Marken, Janice Chen, Van Bao Tran, Quan-zhen Li, Mengtao Li, Karen Cerosaletti, Keith B. Elkon, Xiaofeng Zeng, Natalia V. Giltiay

Research output: Contribution to journalArticle

Abstract

Signaling through Toll-like receptor 7 (TLR7) drives the production of type I IFN and promotes the activation of autoreactive B cells and is implicated in the pathogenesis of systemic lupus erythematosus (SLE). While TLR7 has been extensively studied in murine lupus, much less is known about its role in the pathogenesis of human SLE. Genetic studies support a link between the TLR7 rs3853839 C/G polymorphism, which affects TLR7 mRNA turnover, and SLE susceptibility; however, the effects of this polymorphism on B cells have not been studied. Here we determined how changes in TLR7 expression affect peripheral B cells and auto-Ab production in SLE patients. High TLR7 expression in SLE patients driven by TLR7 rs3853839 C/G polymorphism was associated with more active disease and upregulation of IFN-responsive genes. TLR7hi SLE patients showed an increase in peripheral B cells. Most notably, the percentage and numbers of CD19+CD24++CD38++ newly-formed transitional (TR) B cells were increased in TLR7hi SLE patients as compared to HCs and TLR7norm/lo SLE patients. Using auto-Ab arrays, we found an increase and enrichment of auto-Ab specificities in the TLR7hi SLE group, including the production of anti-RNA/RNP-Abs. Upon in vitro TLR7 ligand stimulation, TR B cells isolated from TLR7hi but not TLR7norm/lo SLE patients produced anti-nuclear auto-Abs (ANA). Exposure of TR B cells isolated from cord blood to IFNα induced the expression of TLR7 and enabled their activation in response to TLR7 ligation in vitro. Our study shows that overexpression of TLR7 in SLE patients drives the expansion of TR B cells. High TLR7 signaling in TR B cells promotes auto-Ab production, supporting a possible pathogenic role of TR B cells in human SLE.

Original languageEnglish (US)
Article number1243
JournalFrontiers in immunology
Volume10
Issue numberJUN
DOIs
StatePublished - Jan 1 2019

Fingerprint

Toll-Like Receptor 7
B-Lymphoid Precursor Cells
Systemic Lupus Erythematosus
Autoantibodies
B-Lymphocytes
Fetal Blood

Keywords

  • Autoantibodies
  • RNP
  • SLE
  • TLR7
  • Transitional B cells
  • Type I IFNs

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

High TLR7 expression drives the expansion of CD19+CD24HiCD38hi transitional B cells and autoantibody production in SLE patients. / Wang, Ting; Marken, John; Chen, Janice; Tran, Van Bao; Li, Quan-zhen; Li, Mengtao; Cerosaletti, Karen; Elkon, Keith B.; Zeng, Xiaofeng; Giltiay, Natalia V.

In: Frontiers in immunology, Vol. 10, No. JUN, 1243, 01.01.2019.

Research output: Contribution to journalArticle

Wang, Ting ; Marken, John ; Chen, Janice ; Tran, Van Bao ; Li, Quan-zhen ; Li, Mengtao ; Cerosaletti, Karen ; Elkon, Keith B. ; Zeng, Xiaofeng ; Giltiay, Natalia V. / High TLR7 expression drives the expansion of CD19+CD24HiCD38hi transitional B cells and autoantibody production in SLE patients. In: Frontiers in immunology. 2019 ; Vol. 10, No. JUN.
@article{5d0f06ca419f4b8196d25e7c4d49c233,
title = "High TLR7 expression drives the expansion of CD19+CD24HiCD38hi transitional B cells and autoantibody production in SLE patients",
abstract = "Signaling through Toll-like receptor 7 (TLR7) drives the production of type I IFN and promotes the activation of autoreactive B cells and is implicated in the pathogenesis of systemic lupus erythematosus (SLE). While TLR7 has been extensively studied in murine lupus, much less is known about its role in the pathogenesis of human SLE. Genetic studies support a link between the TLR7 rs3853839 C/G polymorphism, which affects TLR7 mRNA turnover, and SLE susceptibility; however, the effects of this polymorphism on B cells have not been studied. Here we determined how changes in TLR7 expression affect peripheral B cells and auto-Ab production in SLE patients. High TLR7 expression in SLE patients driven by TLR7 rs3853839 C/G polymorphism was associated with more active disease and upregulation of IFN-responsive genes. TLR7hi SLE patients showed an increase in peripheral B cells. Most notably, the percentage and numbers of CD19+CD24++CD38++ newly-formed transitional (TR) B cells were increased in TLR7hi SLE patients as compared to HCs and TLR7norm/lo SLE patients. Using auto-Ab arrays, we found an increase and enrichment of auto-Ab specificities in the TLR7hi SLE group, including the production of anti-RNA/RNP-Abs. Upon in vitro TLR7 ligand stimulation, TR B cells isolated from TLR7hi but not TLR7norm/lo SLE patients produced anti-nuclear auto-Abs (ANA). Exposure of TR B cells isolated from cord blood to IFNα induced the expression of TLR7 and enabled their activation in response to TLR7 ligation in vitro. Our study shows that overexpression of TLR7 in SLE patients drives the expansion of TR B cells. High TLR7 signaling in TR B cells promotes auto-Ab production, supporting a possible pathogenic role of TR B cells in human SLE.",
keywords = "Autoantibodies, RNP, SLE, TLR7, Transitional B cells, Type I IFNs",
author = "Ting Wang and John Marken and Janice Chen and Tran, {Van Bao} and Quan-zhen Li and Mengtao Li and Karen Cerosaletti and Elkon, {Keith B.} and Xiaofeng Zeng and Giltiay, {Natalia V.}",
year = "2019",
month = "1",
day = "1",
doi = "10.3389/fimmu.2019.01243",
language = "English (US)",
volume = "10",
journal = "Frontiers in Immunology",
issn = "1664-3224",
publisher = "Frontiers Media S. A.",
number = "JUN",

}

TY - JOUR

T1 - High TLR7 expression drives the expansion of CD19+CD24HiCD38hi transitional B cells and autoantibody production in SLE patients

AU - Wang, Ting

AU - Marken, John

AU - Chen, Janice

AU - Tran, Van Bao

AU - Li, Quan-zhen

AU - Li, Mengtao

AU - Cerosaletti, Karen

AU - Elkon, Keith B.

AU - Zeng, Xiaofeng

AU - Giltiay, Natalia V.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Signaling through Toll-like receptor 7 (TLR7) drives the production of type I IFN and promotes the activation of autoreactive B cells and is implicated in the pathogenesis of systemic lupus erythematosus (SLE). While TLR7 has been extensively studied in murine lupus, much less is known about its role in the pathogenesis of human SLE. Genetic studies support a link between the TLR7 rs3853839 C/G polymorphism, which affects TLR7 mRNA turnover, and SLE susceptibility; however, the effects of this polymorphism on B cells have not been studied. Here we determined how changes in TLR7 expression affect peripheral B cells and auto-Ab production in SLE patients. High TLR7 expression in SLE patients driven by TLR7 rs3853839 C/G polymorphism was associated with more active disease and upregulation of IFN-responsive genes. TLR7hi SLE patients showed an increase in peripheral B cells. Most notably, the percentage and numbers of CD19+CD24++CD38++ newly-formed transitional (TR) B cells were increased in TLR7hi SLE patients as compared to HCs and TLR7norm/lo SLE patients. Using auto-Ab arrays, we found an increase and enrichment of auto-Ab specificities in the TLR7hi SLE group, including the production of anti-RNA/RNP-Abs. Upon in vitro TLR7 ligand stimulation, TR B cells isolated from TLR7hi but not TLR7norm/lo SLE patients produced anti-nuclear auto-Abs (ANA). Exposure of TR B cells isolated from cord blood to IFNα induced the expression of TLR7 and enabled their activation in response to TLR7 ligation in vitro. Our study shows that overexpression of TLR7 in SLE patients drives the expansion of TR B cells. High TLR7 signaling in TR B cells promotes auto-Ab production, supporting a possible pathogenic role of TR B cells in human SLE.

AB - Signaling through Toll-like receptor 7 (TLR7) drives the production of type I IFN and promotes the activation of autoreactive B cells and is implicated in the pathogenesis of systemic lupus erythematosus (SLE). While TLR7 has been extensively studied in murine lupus, much less is known about its role in the pathogenesis of human SLE. Genetic studies support a link between the TLR7 rs3853839 C/G polymorphism, which affects TLR7 mRNA turnover, and SLE susceptibility; however, the effects of this polymorphism on B cells have not been studied. Here we determined how changes in TLR7 expression affect peripheral B cells and auto-Ab production in SLE patients. High TLR7 expression in SLE patients driven by TLR7 rs3853839 C/G polymorphism was associated with more active disease and upregulation of IFN-responsive genes. TLR7hi SLE patients showed an increase in peripheral B cells. Most notably, the percentage and numbers of CD19+CD24++CD38++ newly-formed transitional (TR) B cells were increased in TLR7hi SLE patients as compared to HCs and TLR7norm/lo SLE patients. Using auto-Ab arrays, we found an increase and enrichment of auto-Ab specificities in the TLR7hi SLE group, including the production of anti-RNA/RNP-Abs. Upon in vitro TLR7 ligand stimulation, TR B cells isolated from TLR7hi but not TLR7norm/lo SLE patients produced anti-nuclear auto-Abs (ANA). Exposure of TR B cells isolated from cord blood to IFNα induced the expression of TLR7 and enabled their activation in response to TLR7 ligation in vitro. Our study shows that overexpression of TLR7 in SLE patients drives the expansion of TR B cells. High TLR7 signaling in TR B cells promotes auto-Ab production, supporting a possible pathogenic role of TR B cells in human SLE.

KW - Autoantibodies

KW - RNP

KW - SLE

KW - TLR7

KW - Transitional B cells

KW - Type I IFNs

UR - http://www.scopus.com/inward/record.url?scp=85068586008&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068586008&partnerID=8YFLogxK

U2 - 10.3389/fimmu.2019.01243

DO - 10.3389/fimmu.2019.01243

M3 - Article

C2 - 31231380

AN - SCOPUS:85068586008

VL - 10

JO - Frontiers in Immunology

JF - Frontiers in Immunology

SN - 1664-3224

IS - JUN

M1 - 1243

ER -