High translation efficiency is mediated by the encephalomyocarditis virus internal ribosomal entry sites if the natural sequence surrounding the eleventh AUG is retained

Jian Qiao, Vincent Roy, Marie Hélène Girard, Manuel Caruso

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Internal ribosomal entry sites (IRES) allow cap-independent translation and are sequences that are often used in gene therapy for strategies in which several genes need to be expressed. In this study, two different sequences of encephalomyocarditis virus (EMCV) IRES were compared for their translation efficiency in the context of retroviral vectors. When the sequence surrounding the 11th AUG of the IRES was conserved (IRESg), the translation efficiency was significantly higher than if the AUG of the downstream gene started with the 11th AUG of the IRES (IRESb). The translation efficiency with IRESg was influenced by the cell type and also by the nature of the transgene.

Original languageEnglish (US)
Pages (from-to)881-887
Number of pages7
JournalHuman Gene Therapy
Volume13
Issue number7
DOIs
StatePublished - May 1 2002

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Encephalomyocarditis virus
Transgenes
Genetic Therapy
Genes

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

High translation efficiency is mediated by the encephalomyocarditis virus internal ribosomal entry sites if the natural sequence surrounding the eleventh AUG is retained. / Qiao, Jian; Roy, Vincent; Girard, Marie Hélène; Caruso, Manuel.

In: Human Gene Therapy, Vol. 13, No. 7, 01.05.2002, p. 881-887.

Research output: Contribution to journalArticle

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