Hira-dependent histone H3.3 deposition facilitates prc2 recruitment at developmental loci in ES cells

Laura A. Banaszynski, Duancheng Wen, Scott Dewell, Sarah J. Whitcomb, Mingyan Lin, Nichole Diaz, Simon J. Elsässer, Ariane Chapgier, Aaron D. Goldberg, Eli Canaani, Shahin Rafii, Deyou Zheng, C. David Allis

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125 Citations (Scopus)

Abstract

Summary Polycomb repressive complex 2 (PRC2) regulates gene expression during lineage specification through trimethylation of lysine 27 on histone H3 (H3K27me3). In Drosophila, polycomb binding sites are dynamic chromatin regions enriched with the histone variant H3.3. Here, we show that, in mouse embryonic stem cells (ESCs), H3.3 is required for proper establishment of H3K27me3 at the promoters of developmentally regulated genes. Upon H3.3 depletion, these promoters show reduced nucleosome turnover measured by deposition of de novo synthesized histones and reduced PRC2 occupancy. Further, we show H3.3-dependent interaction of PRC2 with the histone chaperone, Hira, and that Hira localization to chromatin requires H3.3. Our data demonstrate the importance of H3.3 in maintaining a chromatin landscape in ESCs that is important for proper gene regulation during differentiation. Moreover, our findings support the emerging notion that H3.3 has multiple functions in distinct genomic locations that are not always correlated with an "active" chromatin state.

Original languageEnglish (US)
Pages (from-to)107-120
Number of pages14
JournalCell
Volume155
Issue number1
DOIs
StatePublished - Sep 26 2013

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Polycomb Repressive Complex 2
Histones
Chromatin
Stem cells
Gene expression
Histone Chaperones
Nucleosomes
Embryonic Stem Cells
Genes
Lysine
Drosophila
Binding Sites
Specifications
Gene Expression

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Hira-dependent histone H3.3 deposition facilitates prc2 recruitment at developmental loci in ES cells. / Banaszynski, Laura A.; Wen, Duancheng; Dewell, Scott; Whitcomb, Sarah J.; Lin, Mingyan; Diaz, Nichole; Elsässer, Simon J.; Chapgier, Ariane; Goldberg, Aaron D.; Canaani, Eli; Rafii, Shahin; Zheng, Deyou; Allis, C. David.

In: Cell, Vol. 155, No. 1, 26.09.2013, p. 107-120.

Research output: Contribution to journalArticle

Banaszynski, LA, Wen, D, Dewell, S, Whitcomb, SJ, Lin, M, Diaz, N, Elsässer, SJ, Chapgier, A, Goldberg, AD, Canaani, E, Rafii, S, Zheng, D & Allis, CD 2013, 'Hira-dependent histone H3.3 deposition facilitates prc2 recruitment at developmental loci in ES cells', Cell, vol. 155, no. 1, pp. 107-120. https://doi.org/10.1016/j.cell.2013.08.061
Banaszynski, Laura A. ; Wen, Duancheng ; Dewell, Scott ; Whitcomb, Sarah J. ; Lin, Mingyan ; Diaz, Nichole ; Elsässer, Simon J. ; Chapgier, Ariane ; Goldberg, Aaron D. ; Canaani, Eli ; Rafii, Shahin ; Zheng, Deyou ; Allis, C. David. / Hira-dependent histone H3.3 deposition facilitates prc2 recruitment at developmental loci in ES cells. In: Cell. 2013 ; Vol. 155, No. 1. pp. 107-120.
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