Histone demethylase KDM6A directly senses oxygen to control chromatin and cell fate

Abhishek A. Chakraborty, Tuomas Laukka, Matti Myllykoski, Alison E. Ringel, Matthew A. Booker, Michael Y. Tolstorukov, Yuzhong Jeff Meng, Samuel R. Meier, Rebecca B. Jennings, Amanda L. Creech, Zachary T. Herbert, Samuel K. McBrayer, Benjamin A. Olenchock, Jacob D. Jaffe, Marcia C. Haigis, Rameen Beroukhim, Sabina Signoretti, Peppi Koivunen, William G. Kaelin

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Oxygen sensing is central to metazoan biology and has implications for human disease. Mammalian cells express multiple oxygen-dependent enzymes called 2-oxoglutarate (OG)-dependent dioxygenases (2-OGDDs), but they vary in their oxygen affinities and hence their ability to sense oxygen. The 2-OGDD histone demethylases control histone methylation. Hypoxia increases histone methylation, but whether this reflects direct effects on histone demethylases or indirect effects caused by the hypoxic induction of the HIF (hypoxia-inducible factor) transcription factor or the 2-OG antagonist 2-hydroxyglutarate (2-HG) is unclear. Here, we report that hypoxia promotes histone methylation in a HIF- and 2-HG–independent manner. We found that the H3K27 histone demethylase KDM6A/UTX, but not its paralog KDM6B, is oxygen sensitive. KDM6A loss, like hypoxia, prevented H3K27 demethylation and blocked cellular differentiation. Restoring H3K27 methylation homeostasis in hypoxic cells reversed these effects. Thus, oxygen directly affects chromatin regulators to control cell fate.

Original languageEnglish (US)
Pages (from-to)1217-1222
Number of pages6
JournalScience
Volume363
Issue number6432
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

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Histone Demethylases
Chromatin
Oxygen
Methylation
Histones
Dioxygenases
Homeostasis
Transcription Factors
Hypoxia
Enzymes

ASJC Scopus subject areas

  • General

Cite this

Chakraborty, A. A., Laukka, T., Myllykoski, M., Ringel, A. E., Booker, M. A., Tolstorukov, M. Y., ... Kaelin, W. G. (2019). Histone demethylase KDM6A directly senses oxygen to control chromatin and cell fate. Science, 363(6432), 1217-1222. https://doi.org/10.1126/science.aaw1026

Histone demethylase KDM6A directly senses oxygen to control chromatin and cell fate. / Chakraborty, Abhishek A.; Laukka, Tuomas; Myllykoski, Matti; Ringel, Alison E.; Booker, Matthew A.; Tolstorukov, Michael Y.; Meng, Yuzhong Jeff; Meier, Samuel R.; Jennings, Rebecca B.; Creech, Amanda L.; Herbert, Zachary T.; McBrayer, Samuel K.; Olenchock, Benjamin A.; Jaffe, Jacob D.; Haigis, Marcia C.; Beroukhim, Rameen; Signoretti, Sabina; Koivunen, Peppi; Kaelin, William G.

In: Science, Vol. 363, No. 6432, 01.01.2019, p. 1217-1222.

Research output: Contribution to journalArticle

Chakraborty, AA, Laukka, T, Myllykoski, M, Ringel, AE, Booker, MA, Tolstorukov, MY, Meng, YJ, Meier, SR, Jennings, RB, Creech, AL, Herbert, ZT, McBrayer, SK, Olenchock, BA, Jaffe, JD, Haigis, MC, Beroukhim, R, Signoretti, S, Koivunen, P & Kaelin, WG 2019, 'Histone demethylase KDM6A directly senses oxygen to control chromatin and cell fate', Science, vol. 363, no. 6432, pp. 1217-1222. https://doi.org/10.1126/science.aaw1026
Chakraborty AA, Laukka T, Myllykoski M, Ringel AE, Booker MA, Tolstorukov MY et al. Histone demethylase KDM6A directly senses oxygen to control chromatin and cell fate. Science. 2019 Jan 1;363(6432):1217-1222. https://doi.org/10.1126/science.aaw1026
Chakraborty, Abhishek A. ; Laukka, Tuomas ; Myllykoski, Matti ; Ringel, Alison E. ; Booker, Matthew A. ; Tolstorukov, Michael Y. ; Meng, Yuzhong Jeff ; Meier, Samuel R. ; Jennings, Rebecca B. ; Creech, Amanda L. ; Herbert, Zachary T. ; McBrayer, Samuel K. ; Olenchock, Benjamin A. ; Jaffe, Jacob D. ; Haigis, Marcia C. ; Beroukhim, Rameen ; Signoretti, Sabina ; Koivunen, Peppi ; Kaelin, William G. / Histone demethylase KDM6A directly senses oxygen to control chromatin and cell fate. In: Science. 2019 ; Vol. 363, No. 6432. pp. 1217-1222.
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