Hiv-1 proviral copy number in blood mononuclear cells from aids patients on zidovudine therapy

R. M. Donovan, R. E. Dickover, E. Goldstein, R. G. Huth, J. R. Carlson

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24 Scopus citations

Abstract

The effect of treatment with 400–1, 200 mg/day of zidovudine (ZDV) on HIVDNA concentrations in patient peripheral blood mononuclear cells (PBMCs) was studied in six patients during a 5- to 14-month period of therapy. HIVDNA was measured in PBMCs at intervals using a recently developed quantitative polymerase chain reaction assay. The amount of HIV DNA ranged from 2, 000 to 40, 000 copies of provirus per microgram of cellular DNA. The HIVprovirus copy number showed little change with time in five patients, and increased and then remained constant in one patient. Thus, prolonged treatment with ZDV does not decrease the levels of HIV DNA in PBMCs.

Original languageEnglish (US)
Pages (from-to)766-769
Number of pages4
JournalJournal of Acquired Immune Deficiency Syndromes
Volume4
Issue number8
StatePublished - Aug 1991

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Keywords

  • Polymerase chain reaction
  • Provirus
  • Zidovudine therapy

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)

Cite this

Donovan, R. M., Dickover, R. E., Goldstein, E., Huth, R. G., & Carlson, J. R. (1991). Hiv-1 proviral copy number in blood mononuclear cells from aids patients on zidovudine therapy. Journal of Acquired Immune Deficiency Syndromes, 4(8), 766-769.