Hiv-1 proviral copy number in blood mononuclear cells from aids patients on zidovudine therapy

R. M. Donovan; R. E. Dickover; E. Goldstein; R. G. Huth; J. R. Carlson

Hiv-1 proviral copy number in blood mononuclear cells from aids patients on zidovudine therapy

R. M. Donovan, R. E. Dickover, E. Goldstein, R. G. Huth, J. R. Carlson

Internal Medicine

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The effect of treatment with 400–1, 200 mg/day of zidovudine (ZDV) on HIVDNA concentrations in patient peripheral blood mononuclear cells (PBMCs) was studied in six patients during a 5- to 14-month period of therapy. HIVDNA was measured in PBMCs at intervals using a recently developed quantitative polymerase chain reaction assay. The amount of HIV DNA ranged from 2, 000 to 40, 000 copies of provirus per microgram of cellular DNA. The HIVprovirus copy number showed little change with time in five patients, and increased and then remained constant in one patient. Thus, prolonged treatment with ZDV does not decrease the levels of HIV DNA in PBMCs.

Original language	English (US)
Pages (from-to)	766-769
Number of pages	4
Journal	Journal of Acquired Immune Deficiency Syndromes
Volume	4
Issue number	8
State	Published - Aug 1991

Keywords

Polymerase chain reaction
Provirus
Zidovudine therapy

ASJC Scopus subject areas

Infectious Diseases
Pharmacology (medical)

Cite this

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title = "Hiv-1 proviral copy number in blood mononuclear cells from aids patients on zidovudine therapy",

abstract = "The effect of treatment with 400–1, 200 mg/day of zidovudine (ZDV) on HIVDNA concentrations in patient peripheral blood mononuclear cells (PBMCs) was studied in six patients during a 5- to 14-month period of therapy. HIVDNA was measured in PBMCs at intervals using a recently developed quantitative polymerase chain reaction assay. The amount of HIV DNA ranged from 2, 000 to 40, 000 copies of provirus per microgram of cellular DNA. The HIVprovirus copy number showed little change with time in five patients, and increased and then remained constant in one patient. Thus, prolonged treatment with ZDV does not decrease the levels of HIV DNA in PBMCs.",

keywords = "Polymerase chain reaction, Provirus, Zidovudine therapy",

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T1 - Hiv-1 proviral copy number in blood mononuclear cells from aids patients on zidovudine therapy

AU - Donovan, R. M.

AU - Dickover, R. E.

AU - Goldstein, E.

AU - Huth, R. G.

AU - Carlson, J. R.

PY - 1991/8

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N2 - The effect of treatment with 400–1, 200 mg/day of zidovudine (ZDV) on HIVDNA concentrations in patient peripheral blood mononuclear cells (PBMCs) was studied in six patients during a 5- to 14-month period of therapy. HIVDNA was measured in PBMCs at intervals using a recently developed quantitative polymerase chain reaction assay. The amount of HIV DNA ranged from 2, 000 to 40, 000 copies of provirus per microgram of cellular DNA. The HIVprovirus copy number showed little change with time in five patients, and increased and then remained constant in one patient. Thus, prolonged treatment with ZDV does not decrease the levels of HIV DNA in PBMCs.

AB - The effect of treatment with 400–1, 200 mg/day of zidovudine (ZDV) on HIVDNA concentrations in patient peripheral blood mononuclear cells (PBMCs) was studied in six patients during a 5- to 14-month period of therapy. HIVDNA was measured in PBMCs at intervals using a recently developed quantitative polymerase chain reaction assay. The amount of HIV DNA ranged from 2, 000 to 40, 000 copies of provirus per microgram of cellular DNA. The HIVprovirus copy number showed little change with time in five patients, and increased and then remained constant in one patient. Thus, prolonged treatment with ZDV does not decrease the levels of HIV DNA in PBMCs.

KW - Polymerase chain reaction

KW - Provirus

KW - Zidovudine therapy

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Hiv-1 proviral copy number in blood mononuclear cells from aids patients on zidovudine therapy

Abstract

Keywords

ASJC Scopus subject areas

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