TY - JOUR
T1 - HLA–D region epitopes associated with juvenile arthritis. recognition by alloreactive t cell clones and alloantisera
AU - Myers, L. K.
AU - Ball, E. J.
AU - Nunez, G.
AU - Fink, C. W.
AU - Stastny, P.
PY - 1987/7
Y1 - 1987/7
N2 - The HLA–D region antigens DR5 (wll, w12), DRw6 (w13,w14), DRw8, DRw52, and DQw1 have previously been shown to be increased in frequency in subsets of patients with juvenile arthritis. Since the HLA–D region is complex (composed of at least 3 subregions encoding multiple molecules, each in turn presenting multiple alloantigenic epitopes), we sought to clarify whether one strongly associated factor might explain the previous findings. To search for the pertinent HLA–D region stimulatory epitopes, alloreactive T cells were primed against DR5 and DRw6 haplotypes and cloned by limiting dilution. Three T cell clones and 1 alloantiserum met the criteria for significant association with juvenile arthritis on patient testing, including DR5, DRw6, and DRw8 haplotypes. Monoclonal antibody blocking revealed that all 4 recognized epitopes on DR subregion products. For 2 of the clones, the relative risks for JA (10.5 and 9.4) were higher than the risks with any other previously described typing reagents.
AB - The HLA–D region antigens DR5 (wll, w12), DRw6 (w13,w14), DRw8, DRw52, and DQw1 have previously been shown to be increased in frequency in subsets of patients with juvenile arthritis. Since the HLA–D region is complex (composed of at least 3 subregions encoding multiple molecules, each in turn presenting multiple alloantigenic epitopes), we sought to clarify whether one strongly associated factor might explain the previous findings. To search for the pertinent HLA–D region stimulatory epitopes, alloreactive T cells were primed against DR5 and DRw6 haplotypes and cloned by limiting dilution. Three T cell clones and 1 alloantiserum met the criteria for significant association with juvenile arthritis on patient testing, including DR5, DRw6, and DRw8 haplotypes. Monoclonal antibody blocking revealed that all 4 recognized epitopes on DR subregion products. For 2 of the clones, the relative risks for JA (10.5 and 9.4) were higher than the risks with any other previously described typing reagents.
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U2 - 10.1002/art.1780300704
DO - 10.1002/art.1780300704
M3 - Article
C2 - 2441709
AN - SCOPUS:0023159949
SN - 0004-3591
VL - 30
SP - 744
EP - 751
JO - Arthritis & Rheumatism
JF - Arthritis & Rheumatism
IS - 7
ER -