HL-D region epitopes associated with juvenile arthritis. Recognition by alloreactive T cell clones and alloantisera

L. K. Myers, E. J. Ball, G. Nunez, C. W. Fink, P. Stastny

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9 Citations (Scopus)

Abstract

The HLA-D region antigens DR5 (w11, w12), DRw6 (w13,w14), DRw8, DRw52, and DQw1 have previously been shown to be increased in frequency in subsets of patients with juvenile arthritis. Since the HLA-D region is complex (composed of at least 3 subregions encoding multiple molecules, each in turn presenting multiple alloantigenic epitopes), we sought to clarify whether one strongly associated factor might explain the previous findings. To search for the pertinent HLA-D region stimulatory epitopes, alloreactive T cells were primed against DR5 and DRw6 haplotypes and cloned by limiting dilution. Three T cell clones and 1 alloantiserum met the criteria for significant association with juvenile arthritis on patient testing, including DR5, DRw6, and DRw8 haplotypes. Monoclonal antibody blocking revealed that all 4 recognized epitopes on DR subregion products. For 2 of the clones, the relative risks for JA (10.5 and 9.4) were higher than the risks with any other previously described typing reagents.

Original languageEnglish (US)
Pages (from-to)744-751
Number of pages8
JournalArthritis and Rheumatism
Volume30
Issue number7
StatePublished - 1987

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HLA-D Antigens
Juvenile Arthritis
Epitopes
Clone Cells
T-Lymphocytes
Haplotypes
T-Lymphocyte Epitopes
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Myers, L. K., Ball, E. J., Nunez, G., Fink, C. W., & Stastny, P. (1987). HL-D region epitopes associated with juvenile arthritis. Recognition by alloreactive T cell clones and alloantisera. Arthritis and Rheumatism, 30(7), 744-751.

HL-D region epitopes associated with juvenile arthritis. Recognition by alloreactive T cell clones and alloantisera. / Myers, L. K.; Ball, E. J.; Nunez, G.; Fink, C. W.; Stastny, P.

In: Arthritis and Rheumatism, Vol. 30, No. 7, 1987, p. 744-751.

Research output: Contribution to journalArticle

Myers, LK, Ball, EJ, Nunez, G, Fink, CW & Stastny, P 1987, 'HL-D region epitopes associated with juvenile arthritis. Recognition by alloreactive T cell clones and alloantisera', Arthritis and Rheumatism, vol. 30, no. 7, pp. 744-751.
Myers, L. K. ; Ball, E. J. ; Nunez, G. ; Fink, C. W. ; Stastny, P. / HL-D region epitopes associated with juvenile arthritis. Recognition by alloreactive T cell clones and alloantisera. In: Arthritis and Rheumatism. 1987 ; Vol. 30, No. 7. pp. 744-751.
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