HLA-A, B, C; -DR; -MT, -MB, and SB antigens on unstimulated human endothelial cells

J. Neppert, G. Nunez, P. Stastny

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Since major histocompatibility complex (MHC) angigens of class II play an important role in organ transplantation, we attempted to demonstrate their expression on endothelial cells which are abundant in transplants with vascular blood supply. Our analysis was performed with monomorphic and polymorphic monoclonal antibodies employing a microscopic immunofluorescence assay and flow cytometry on unstimulated endothelial cells isolated from umbilical cord veins. No evidence was found for the presence of HLA-DR antigens and determinants associated with MT, MB and SB. MHC class I antigens exhibited reduced expression. These findings were confirmed in respect to alloantigens by the use of conventional cytotoxic tissue typing antisera. MB and SB antigens were present on most cord B lymphocytes, but could only be demonstrated on a subpopulation of monocytes exhibiting a lower antigen density at the cell surface. MT and DR antigens were found on most cord monocytes and B lymphocytes.

Original languageEnglish (US)
Pages (from-to)40-47
Number of pages8
JournalTissue Antigens
Volume24
Issue number1
StatePublished - 1984

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HLA-A Antigens
HLA-B Antigens
Endothelial Cells
Major Histocompatibility Complex
Antigens
Monocytes
B-Lymphocytes
Histocompatibility Testing
Histocompatibility Antigens Class I
Umbilical Veins
Isoantigens
Umbilical Cord
Organ Transplantation
HLA-DR Antigens
Fluorescent Antibody Technique
Blood Vessels
Immune Sera
Flow Cytometry
Cell Count
Monoclonal Antibodies

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

HLA-A, B, C; -DR; -MT, -MB, and SB antigens on unstimulated human endothelial cells. / Neppert, J.; Nunez, G.; Stastny, P.

In: Tissue Antigens, Vol. 24, No. 1, 1984, p. 40-47.

Research output: Contribution to journalArticle

Neppert, J, Nunez, G & Stastny, P 1984, 'HLA-A, B, C; -DR; -MT, -MB, and SB antigens on unstimulated human endothelial cells', Tissue Antigens, vol. 24, no. 1, pp. 40-47.
Neppert, J. ; Nunez, G. ; Stastny, P. / HLA-A, B, C; -DR; -MT, -MB, and SB antigens on unstimulated human endothelial cells. In: Tissue Antigens. 1984 ; Vol. 24, No. 1. pp. 40-47.
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