HLA loss variants of a B27+ lymphoblastoid cell line: Genetic and cellular characterization

Janice A. Nicklas, Yoshiki Miyachi, Joel D. Taurog, Siew Lin Wee, Li Kuang Chen, F. Carl Grumet, Fritz H. Bach

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Variants of a lymphoblastoid cell line, LCL 526 (SB3 MB1 DR1 B44 C5 A2/SB4 MT4 DR4 B27 C2 A24), which lost various HLA specificities were selected with monoclonal antibodies and complement using a method developed by Kavathas et al. (PNAS 77:4251, 1980). Using αB27 monoclonals, 8 B27 only loss mutants and 4 B27 haplotype multiple loss mutants were generated. The parental LCL 526 and two of the B27- mutants were used to select αB27 CTLs. The selection of six A2 loss, one A2-C5 loss, and 14 A2 haplotype multiple loss variants as well as secondary selection on haplotype loss variants to obtain A null, B null, DR null, and total A,B,C, null variants is also described. The usefulness of these mutants for the study of the relationship between B27 and disease and as two new haplotypes for immunologic, genetic, and molecular research is discussed. These mutants are available to other researchers.

Original languageEnglish (US)
Pages (from-to)19-30
Number of pages12
JournalHuman Immunology
Volume11
Issue number1
DOIs
StatePublished - 1984

Fingerprint

varespladib methyl
Haplotypes
Cell Line
Genetic Research
Monoclonal Antibodies
Research Personnel

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

HLA loss variants of a B27+ lymphoblastoid cell line : Genetic and cellular characterization. / Nicklas, Janice A.; Miyachi, Yoshiki; Taurog, Joel D.; Wee, Siew Lin; Chen, Li Kuang; Grumet, F. Carl; Bach, Fritz H.

In: Human Immunology, Vol. 11, No. 1, 1984, p. 19-30.

Research output: Contribution to journalArticle

Nicklas, Janice A. ; Miyachi, Yoshiki ; Taurog, Joel D. ; Wee, Siew Lin ; Chen, Li Kuang ; Grumet, F. Carl ; Bach, Fritz H. / HLA loss variants of a B27+ lymphoblastoid cell line : Genetic and cellular characterization. In: Human Immunology. 1984 ; Vol. 11, No. 1. pp. 19-30.
@article{db1a260ac6e3474d87b55d9fed18277d,
title = "HLA loss variants of a B27+ lymphoblastoid cell line: Genetic and cellular characterization",
abstract = "Variants of a lymphoblastoid cell line, LCL 526 (SB3 MB1 DR1 B44 C5 A2/SB4 MT4 DR4 B27 C2 A24), which lost various HLA specificities were selected with monoclonal antibodies and complement using a method developed by Kavathas et al. (PNAS 77:4251, 1980). Using αB27 monoclonals, 8 B27 only loss mutants and 4 B27 haplotype multiple loss mutants were generated. The parental LCL 526 and two of the B27- mutants were used to select αB27 CTLs. The selection of six A2 loss, one A2-C5 loss, and 14 A2 haplotype multiple loss variants as well as secondary selection on haplotype loss variants to obtain A null, B null, DR null, and total A,B,C, null variants is also described. The usefulness of these mutants for the study of the relationship between B27 and disease and as two new haplotypes for immunologic, genetic, and molecular research is discussed. These mutants are available to other researchers.",
author = "Nicklas, {Janice A.} and Yoshiki Miyachi and Taurog, {Joel D.} and Wee, {Siew Lin} and Chen, {Li Kuang} and Grumet, {F. Carl} and Bach, {Fritz H.}",
year = "1984",
doi = "10.1016/0198-8859(84)90053-3",
language = "English (US)",
volume = "11",
pages = "19--30",
journal = "Human Immunology",
issn = "0198-8859",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - HLA loss variants of a B27+ lymphoblastoid cell line

T2 - Genetic and cellular characterization

AU - Nicklas, Janice A.

AU - Miyachi, Yoshiki

AU - Taurog, Joel D.

AU - Wee, Siew Lin

AU - Chen, Li Kuang

AU - Grumet, F. Carl

AU - Bach, Fritz H.

PY - 1984

Y1 - 1984

N2 - Variants of a lymphoblastoid cell line, LCL 526 (SB3 MB1 DR1 B44 C5 A2/SB4 MT4 DR4 B27 C2 A24), which lost various HLA specificities were selected with monoclonal antibodies and complement using a method developed by Kavathas et al. (PNAS 77:4251, 1980). Using αB27 monoclonals, 8 B27 only loss mutants and 4 B27 haplotype multiple loss mutants were generated. The parental LCL 526 and two of the B27- mutants were used to select αB27 CTLs. The selection of six A2 loss, one A2-C5 loss, and 14 A2 haplotype multiple loss variants as well as secondary selection on haplotype loss variants to obtain A null, B null, DR null, and total A,B,C, null variants is also described. The usefulness of these mutants for the study of the relationship between B27 and disease and as two new haplotypes for immunologic, genetic, and molecular research is discussed. These mutants are available to other researchers.

AB - Variants of a lymphoblastoid cell line, LCL 526 (SB3 MB1 DR1 B44 C5 A2/SB4 MT4 DR4 B27 C2 A24), which lost various HLA specificities were selected with monoclonal antibodies and complement using a method developed by Kavathas et al. (PNAS 77:4251, 1980). Using αB27 monoclonals, 8 B27 only loss mutants and 4 B27 haplotype multiple loss mutants were generated. The parental LCL 526 and two of the B27- mutants were used to select αB27 CTLs. The selection of six A2 loss, one A2-C5 loss, and 14 A2 haplotype multiple loss variants as well as secondary selection on haplotype loss variants to obtain A null, B null, DR null, and total A,B,C, null variants is also described. The usefulness of these mutants for the study of the relationship between B27 and disease and as two new haplotypes for immunologic, genetic, and molecular research is discussed. These mutants are available to other researchers.

UR - http://www.scopus.com/inward/record.url?scp=0021612210&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021612210&partnerID=8YFLogxK

U2 - 10.1016/0198-8859(84)90053-3

DO - 10.1016/0198-8859(84)90053-3

M3 - Article

C2 - 6090351

AN - SCOPUS:0021612210

VL - 11

SP - 19

EP - 30

JO - Human Immunology

JF - Human Immunology

SN - 0198-8859

IS - 1

ER -