HLA‐A, B, C; ‐DR; ‐MT, ‐MB, and SB antigens on unstimulated human endothelial cells

J. Neppert, G. Nunez, P. Stastny

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Since major histocompatibility complex (MHC) antigens of class II play an important role in organ transplantation, we attempted to demonstrate their expression on endothelial cells which are abundant in transplants with vascular blood supply. Our analysis was performed with monomorphic and polymorphic monoclonal antibodies employing a microscopic immunofluorescence assay and flow cytometry on unstimulated endothelial cells isolated from umbilical cord veins. No evidence was found for the presence of HLA‐DR antigens and determinants associated with MT, MB and SB. MHC class I antigens exhibited reduced expression. These findings were confirmed in respect to alloantigens by the use of conventional cytotoxic tissue typing antisera. MB and SB antigens were present on most cord B lymphocytes, but could only be demonstrated on a subpopulation of monocytes exhibiting a lower antigen density at the cell surface. MT and DR antigens were found on most cord monocytes and B lymphocytes.

Original languageEnglish (US)
Pages (from-to)40-47
Number of pages8
JournalTissue Antigens
Volume24
Issue number1
DOIs
StatePublished - Jul 1984

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Genetics

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