HLA–D region epitopes associated with juvenile arthritis. recognition by alloreactive t cell clones and alloantisera

L. K. Myers, E. J. Ball, G. Nunez, C. W. Fink, P. Stastny

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The HLA–D region antigens DR5 (wll, w12), DRw6 (w13,w14), DRw8, DRw52, and DQw1 have previously been shown to be increased in frequency in subsets of patients with juvenile arthritis. Since the HLA–D region is complex (composed of at least 3 subregions encoding multiple molecules, each in turn presenting multiple alloantigenic epitopes), we sought to clarify whether one strongly associated factor might explain the previous findings. To search for the pertinent HLA–D region stimulatory epitopes, alloreactive T cells were primed against DR5 and DRw6 haplotypes and cloned by limiting dilution. Three T cell clones and 1 alloantiserum met the criteria for significant association with juvenile arthritis on patient testing, including DR5, DRw6, and DRw8 haplotypes. Monoclonal antibody blocking revealed that all 4 recognized epitopes on DR subregion products. For 2 of the clones, the relative risks for JA (10.5 and 9.4) were higher than the risks with any other previously described typing reagents.

Original languageEnglish (US)
Pages (from-to)744-751
Number of pages8
JournalArthritis & Rheumatism
Volume30
Issue number7
DOIs
StatePublished - Jul 1987

ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'HLA–D region epitopes associated with juvenile arthritis. recognition by alloreactive t cell clones and alloantisera'. Together they form a unique fingerprint.

  • Cite this