Hook2 contributes to aggresome formation

Györgyi Szebenyi, W. Christian Wigley, Branden Hall, Aaron Didier, Michelle Yu, Philip Thomas, Helmut Krämer

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Background: Aggresomes are pericentrosomal accumulations of misfolded proteins, chaperones and proteasomes. Their positioning near the centrosome, like that of other organelles, requires active, microtubule-dependent transport. Linker proteins that can associate with the motor protein dynein, organelles, and microtubules are thought to contribute to the active maintenance of the juxtanuclear localization of many membrane bound organelles and aggresomes. Hook proteins have been proposed to serve as adaptors for the association of cargos with dynein for transport on microtubules. Hook2 was shown to localize to the centrosome, bind centriolin, and contribute to centrosomal function. Results: Here we show that overexpression of hook2 promotes the accumulation of the cystic fibrosis transmembrane regulator in aggresomes without altering its biochemical properties or its steady state level. A dominant negatively acting form of hook2 that lacks the centriolin binding C-terminal inhibits aggresome formation. Conclusion: We propose that hook2 contributes to the establishment and maintenance of the pericentrosomal localization of aggresomes by promoting the microtubule-based delivery of protein aggregates to pericentriolar aggresomes.

Original languageEnglish (US)
Article number19
JournalBMC Cell Biology
Volume8
DOIs
StatePublished - May 31 2007

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Microtubules
Organelles
Dyneins
Centrosome
Proteins
Maintenance
Proteasome Endopeptidase Complex
Cystic Fibrosis
Membranes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Hook2 contributes to aggresome formation. / Szebenyi, Györgyi; Wigley, W. Christian; Hall, Branden; Didier, Aaron; Yu, Michelle; Thomas, Philip; Krämer, Helmut.

In: BMC Cell Biology, Vol. 8, 19, 31.05.2007.

Research output: Contribution to journalArticle

Szebenyi G, Wigley WC, Hall B, Didier A, Yu M, Thomas P et al. Hook2 contributes to aggresome formation. BMC Cell Biology. 2007 May 31;8. 19. https://doi.org/10.1186/1471-2121-8-19
Szebenyi, Györgyi ; Wigley, W. Christian ; Hall, Branden ; Didier, Aaron ; Yu, Michelle ; Thomas, Philip ; Krämer, Helmut. / Hook2 contributes to aggresome formation. In: BMC Cell Biology. 2007 ; Vol. 8.
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