Hook2 is involved in the morphogenesis of the primary cilium

Carole L. Baron Gaillard, Emilie Pallesi-Pocachard, Dominique Massey-Harroche, Fabrice Richard, Jean Pierre Arsanto, Jean Paul Chauvin, Patrick Lecine, Helmut Krämer, Jean Paul Borg, André Le Bivic

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Primary cilia originate from the centrosome and play essential roles in several cellular, developmental, and pathological processes, but the underlying mechanisms of ciliogenesis are not fully understood. Given the involvement of the adaptor protein Hook2 in centrosomal homeostasis and protein transport to pericentrosomal aggresomes, we explored its role in ciliogenesis. We found that in human retinal epithelial cells, Hook2 localizes at the Golgi apparatus and centrosome/basal body, a strategic partitioning for ciliogenesis. Of importance, Hook2 depletion disrupts ciliogenesis at a stage before the formation of the ciliary vesicle at the distal tip of the mother centriole. Using two hybrid and immunoprecipitation assays and a small interfering RNA strategy, we found that Hook2 interacts with and stabilizes pericentriolar material protein 1 (PCM1), which was reported to be essential for the recruitment of Rab8a, a GTPase that is believed to be crucial for membrane transport to the primary cilium. Of interest, GFP::Rab8a coimmunoprecipitates with endogenous Hook2 and PCM1. Finally, GFP::Rab8a can overcome Hook2 depletion, demonstrating a functional interaction between Hook2 and these two important regulators of ciliogenesis. The data indicate that Hook2 interacts with PCM1 in a complex that also contains Rab8a and regulates a limiting step required for further initiation of ciliogenesis after centriole maturation.

Original languageEnglish (US)
Pages (from-to)4549-4562
Number of pages14
JournalMolecular Biology of the Cell
Volume22
Issue number23
DOIs
StatePublished - Dec 1 2011

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Cilia
Morphogenesis
Centrioles
Centrosome
Proteins
Basal Bodies
Two-Hybrid System Techniques
GTP Phosphohydrolases
Golgi Apparatus
Protein Transport
Pathologic Processes
Immunoprecipitation
Small Interfering RNA
Homeostasis
Epithelial Cells
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Baron Gaillard, C. L., Pallesi-Pocachard, E., Massey-Harroche, D., Richard, F., Arsanto, J. P., Chauvin, J. P., ... Le Bivic, A. (2011). Hook2 is involved in the morphogenesis of the primary cilium. Molecular Biology of the Cell, 22(23), 4549-4562. https://doi.org/10.1091/mbc.E11-05-0405

Hook2 is involved in the morphogenesis of the primary cilium. / Baron Gaillard, Carole L.; Pallesi-Pocachard, Emilie; Massey-Harroche, Dominique; Richard, Fabrice; Arsanto, Jean Pierre; Chauvin, Jean Paul; Lecine, Patrick; Krämer, Helmut; Borg, Jean Paul; Le Bivic, André.

In: Molecular Biology of the Cell, Vol. 22, No. 23, 01.12.2011, p. 4549-4562.

Research output: Contribution to journalArticle

Baron Gaillard, CL, Pallesi-Pocachard, E, Massey-Harroche, D, Richard, F, Arsanto, JP, Chauvin, JP, Lecine, P, Krämer, H, Borg, JP & Le Bivic, A 2011, 'Hook2 is involved in the morphogenesis of the primary cilium', Molecular Biology of the Cell, vol. 22, no. 23, pp. 4549-4562. https://doi.org/10.1091/mbc.E11-05-0405
Baron Gaillard CL, Pallesi-Pocachard E, Massey-Harroche D, Richard F, Arsanto JP, Chauvin JP et al. Hook2 is involved in the morphogenesis of the primary cilium. Molecular Biology of the Cell. 2011 Dec 1;22(23):4549-4562. https://doi.org/10.1091/mbc.E11-05-0405
Baron Gaillard, Carole L. ; Pallesi-Pocachard, Emilie ; Massey-Harroche, Dominique ; Richard, Fabrice ; Arsanto, Jean Pierre ; Chauvin, Jean Paul ; Lecine, Patrick ; Krämer, Helmut ; Borg, Jean Paul ; Le Bivic, André. / Hook2 is involved in the morphogenesis of the primary cilium. In: Molecular Biology of the Cell. 2011 ; Vol. 22, No. 23. pp. 4549-4562.
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