Hook2 localizes to the centrosome, binds directly to centriolin/CEP110 and contributes to centrosomal function

Györgyi Szebenyi, Branden Hall, Rosa Yu, Arig Ibrahim Hashim, Helmut Krämer

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Centrosomes serve as microtubule-organizing centers. However, centrosome function depends on microtubule organization and protein transport because the formation, positioning and maintenance of centrosomes require microtubule-dependent retrograde transport. Linker proteins that associate with the motor protein dynein, organelles and microtubules facilitate loading of cargos for retrograde transport and thus contribute to the composition and placement of the centrosome and other juxtanuclear protein complexes. Members of the hook family of proteins may function as adaptors to link various organelle cargos to dynein for transport and have also been implicated directly in centrosome positioning. Here, we show that mammalian hook2, a previously uncharacterized member of the hook family, localizes to the centrosome through all phases of the cell cycle, the C-terminal domain of hook2 directly binds to centriolin/CEP110, the expression of the C-terminal domain of centriolin/CEP110 alters the distribution of endogenous hook2 and mislocalized wild-type or mutant hook2 proteins perturb endogenous centrosomal and pericentrosomal proteins in cultured mammalian cells. In addition, interference with hook2 function results in the loss of the radial organization of microtubules and a defect in regrowth of microtubules following their nocodazole-induced depolymerization. Thus, we propose that hook2 contributes to the establishment and maintenance of centrosomal structure and function.

Original languageEnglish (US)
Pages (from-to)32-46
Number of pages15
JournalTraffic
Volume8
Issue number1
DOIs
StatePublished - Jan 2007

Fingerprint

Centrosome
Microtubules
Dyneins
Proteins
Hooks
Organelles
Maintenance
Microtubule-Organizing Center
Microtubule Proteins
Nocodazole
Cells
Protein Transport
Mutant Proteins
Depolymerization
Cultured Cells
Cell Cycle
Defects
Chemical analysis

Keywords

  • Cell cycle
  • Centriolin/CEP110
  • Centrosome
  • Microtubule organization
  • Organelle positioning

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Structural Biology
  • Molecular Biology
  • Genetics

Cite this

Hook2 localizes to the centrosome, binds directly to centriolin/CEP110 and contributes to centrosomal function. / Szebenyi, Györgyi; Hall, Branden; Yu, Rosa; Hashim, Arig Ibrahim; Krämer, Helmut.

In: Traffic, Vol. 8, No. 1, 01.2007, p. 32-46.

Research output: Contribution to journalArticle

Szebenyi, Györgyi ; Hall, Branden ; Yu, Rosa ; Hashim, Arig Ibrahim ; Krämer, Helmut. / Hook2 localizes to the centrosome, binds directly to centriolin/CEP110 and contributes to centrosomal function. In: Traffic. 2007 ; Vol. 8, No. 1. pp. 32-46.
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