Hormonal control of cardiac protein and amino acid balance

K. Wildenthal, E. E. Griffin, J. S. Ingwall

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Isolated hearts of fetal mice in organ culture maintain active protein synthesis and protein degradation. Rates of degradation exceed rates of synthesis and, as a result, the hearts are in a state of negative protein balance as evidenced by net loss of protein and release of amino acids. Several hormones can alter amino acid metabolism and protein balance by altering synthesis or degradation or both. In cultured fetal mouse hearts, insulin, the most extensively studied of the hormones, increases the rate of protein synthesis by 13 ± 3.7 % and decreases the rate of protein degradation by 22 ± 4.1 % (P < 0.01 for both). Together, these changes account for a 30-40 % reduction in the loss of cardiac protein and in the release of phenylalanine from the heart. These changes are accompanied by a decrease of 21 ± 2.7 % in the total activity of the lysosomal proteinase cathepsin D, and by a reduction in the proportion of the enzyme that is present in the nonsedimentable fraction of the issue homogenate. This suggests the possibility that insulin may function in part by altering lysosomal enzyme activity or availability, or both. The effects of insulin on protein degradation, amino acid release, and cathepsin D activity persist even when protein synthesis has been inhibited by cycloheximide. These results suggest that insulin plays an important role in the control of cardiac protein synthesis and degradation.

Original languageEnglish (US)
Pages (from-to)138-144
Number of pages7
JournalCirculation Research
Volume38
Issue number5 sup 1
StatePublished - 1976

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Amino Acids
Proteolysis
Proteins
Insulin
Fetal Heart
Cathepsin D
Hormones
Organ Culture Techniques
Enzymes
Cycloheximide
Phenylalanine

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Wildenthal, K., Griffin, E. E., & Ingwall, J. S. (1976). Hormonal control of cardiac protein and amino acid balance. Circulation Research, 38(5 sup 1), 138-144.

Hormonal control of cardiac protein and amino acid balance. / Wildenthal, K.; Griffin, E. E.; Ingwall, J. S.

In: Circulation Research, Vol. 38, No. 5 sup 1, 1976, p. 138-144.

Research output: Contribution to journalArticle

Wildenthal, K, Griffin, EE & Ingwall, JS 1976, 'Hormonal control of cardiac protein and amino acid balance', Circulation Research, vol. 38, no. 5 sup 1, pp. 138-144.
Wildenthal K, Griffin EE, Ingwall JS. Hormonal control of cardiac protein and amino acid balance. Circulation Research. 1976;38(5 sup 1):138-144.
Wildenthal, K. ; Griffin, E. E. ; Ingwall, J. S. / Hormonal control of cardiac protein and amino acid balance. In: Circulation Research. 1976 ; Vol. 38, No. 5 sup 1. pp. 138-144.
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