TY - JOUR
T1 - Hormonal regulation of the synthesis of lamellar body phosphatidylglycerol and phosphatidylinositol in fetal lung tissue
AU - Snyder, J. M.
AU - Longmuir, K. J.
AU - Johnston, J. M.
AU - Mendelson, C. R.
PY - 1983/3
Y1 - 1983/3
N2 - The influence of hormones on the synthesis of phospholipids of lung lamellar bodies was studied using lung tissue explants from 16- to 22-week gestational age human abortuses. Lung explants were incubated in a defined medium containing either no hormonal additions or insulin (2.5μg/ml), cortisol (0.2 μg/ml), or PRL (2.5 μg/ml)> added alone or in various combinations. After 7 days of incubation, a purified lamellar body fraction was isolated from the explants. The effects of various hormone combinations on the relative rates of synthesis of lamellar body anionic glycerophospholipids during the last 24 h of culture were analyzed and compared to that of control explants. In control explants, 7.1% of the glycerophospholipid was synthesized as phosphatidylinositol, and 2.2% was synthesized as phosphatidylglycerol. Phosphatidylinositol synthesis was decreased significantly in explants incubated with cortisol alone, insulin plus cortisol, or insulin plus cortisol plus PRL (to 4.4%, 4.3%, and 3.7%, respectively), while phosphatidylglycerol synthesis was increased significantly in lamellar bodies from explants incubated with insulin plus cortisol or insulin plus cortisol plus PRL (to 5.5% and 5.7%, respectively). Although the relative rates of synthesis of lamellar body phosphatidylinositol and phosphatidylglycerol were significantly altered by hormones, the percentages of the total anionic glycerophospholipid were similar in all treatment groups (9.5%). The results of this study, therefore, are suggestive that lamellar body formation and glycerophospholipid composition are under hormonal control.
AB - The influence of hormones on the synthesis of phospholipids of lung lamellar bodies was studied using lung tissue explants from 16- to 22-week gestational age human abortuses. Lung explants were incubated in a defined medium containing either no hormonal additions or insulin (2.5μg/ml), cortisol (0.2 μg/ml), or PRL (2.5 μg/ml)> added alone or in various combinations. After 7 days of incubation, a purified lamellar body fraction was isolated from the explants. The effects of various hormone combinations on the relative rates of synthesis of lamellar body anionic glycerophospholipids during the last 24 h of culture were analyzed and compared to that of control explants. In control explants, 7.1% of the glycerophospholipid was synthesized as phosphatidylinositol, and 2.2% was synthesized as phosphatidylglycerol. Phosphatidylinositol synthesis was decreased significantly in explants incubated with cortisol alone, insulin plus cortisol, or insulin plus cortisol plus PRL (to 4.4%, 4.3%, and 3.7%, respectively), while phosphatidylglycerol synthesis was increased significantly in lamellar bodies from explants incubated with insulin plus cortisol or insulin plus cortisol plus PRL (to 5.5% and 5.7%, respectively). Although the relative rates of synthesis of lamellar body phosphatidylinositol and phosphatidylglycerol were significantly altered by hormones, the percentages of the total anionic glycerophospholipid were similar in all treatment groups (9.5%). The results of this study, therefore, are suggestive that lamellar body formation and glycerophospholipid composition are under hormonal control.
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U2 - 10.1210/endo-112-3-1012
DO - 10.1210/endo-112-3-1012
M3 - Article
C2 - 6337040
AN - SCOPUS:0020725245
SN - 0013-7227
VL - 112
SP - 1012
EP - 1018
JO - Endocrinology
JF - Endocrinology
IS - 3
ER -