Hormonal regulation of the synthesis of lamellar body phosphatidylglycerol and phosphatidylinositol in fetal lung tissue.

J. M. Snyder, K. J. Longmuir, J. M. Johnston, C. R. Mendelson

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The influence of hormones on the synthesis of phospholipids of lung lamellar bodies was studied using lung tissue explants from 16- to 22-week gestational age human abortuses. Lung explants were incubated in a defined medium containing either no hormonal additions or insulin (2.5 micrograms/ml), cortisol (0.2 microgram/ml), or PRL (2.5 micrograms/ml), added alone or in various combinations. After 7 days of incubation, a purified lamellar body fraction was isolated from the explants. The effects of various hormone combinations on the relative rates of synthesis of lamellar body anionic glycerophospholipids during the last 24 h of culture were analyzed and compared to that of control explants. In control explants, 7.1% of the glycerophospholipid was synthesized as phosphatidylinositol, and 2.2% was synthesized as phosphatidylglycerol. Phosphatidylinositol synthesis was decreased significantly in explants incubated with cortisol alone, insulin plus cortisol, or insulin plus cortisol plus PRL (to 4.4%, 4.3%, and 3.7%, respectively), while phosphatidylglycerol synthesis was increased significantly in lamellar bodies from explants incubated with insulin plus cortisol or insulin plus cortisol plus PRL (to 5.5% and 5.7%, respectively). Although the relative rates of synthesis of lamellar body phosphatidylinositol and phosphatidylglycerol were significantly altered by hormones, the percentages of the total anionic glycerophospholipid were similar in all treatment groups (9.5%). The results of this study, therefore, are suggestive that lamellar body formation and glycerophospholipid composition are under hormonal control.

Original languageEnglish (US)
Pages (from-to)1012-1018
Number of pages7
JournalEndocrinology
Volume112
Issue number3
StatePublished - Mar 1983

Fingerprint

Phosphatidylglycerols
Phosphatidylinositols
Glycerophospholipids
Hydrocortisone
Fetus
Lung
Insulin
Hormones
Gestational Age
Phospholipids

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Hormonal regulation of the synthesis of lamellar body phosphatidylglycerol and phosphatidylinositol in fetal lung tissue. / Snyder, J. M.; Longmuir, K. J.; Johnston, J. M.; Mendelson, C. R.

In: Endocrinology, Vol. 112, No. 3, 03.1983, p. 1012-1018.

Research output: Contribution to journalArticle

@article{7fe9d7ddbb764223999e3c3a08950b93,
title = "Hormonal regulation of the synthesis of lamellar body phosphatidylglycerol and phosphatidylinositol in fetal lung tissue.",
abstract = "The influence of hormones on the synthesis of phospholipids of lung lamellar bodies was studied using lung tissue explants from 16- to 22-week gestational age human abortuses. Lung explants were incubated in a defined medium containing either no hormonal additions or insulin (2.5 micrograms/ml), cortisol (0.2 microgram/ml), or PRL (2.5 micrograms/ml), added alone or in various combinations. After 7 days of incubation, a purified lamellar body fraction was isolated from the explants. The effects of various hormone combinations on the relative rates of synthesis of lamellar body anionic glycerophospholipids during the last 24 h of culture were analyzed and compared to that of control explants. In control explants, 7.1{\%} of the glycerophospholipid was synthesized as phosphatidylinositol, and 2.2{\%} was synthesized as phosphatidylglycerol. Phosphatidylinositol synthesis was decreased significantly in explants incubated with cortisol alone, insulin plus cortisol, or insulin plus cortisol plus PRL (to 4.4{\%}, 4.3{\%}, and 3.7{\%}, respectively), while phosphatidylglycerol synthesis was increased significantly in lamellar bodies from explants incubated with insulin plus cortisol or insulin plus cortisol plus PRL (to 5.5{\%} and 5.7{\%}, respectively). Although the relative rates of synthesis of lamellar body phosphatidylinositol and phosphatidylglycerol were significantly altered by hormones, the percentages of the total anionic glycerophospholipid were similar in all treatment groups (9.5{\%}). The results of this study, therefore, are suggestive that lamellar body formation and glycerophospholipid composition are under hormonal control.",
author = "Snyder, {J. M.} and Longmuir, {K. J.} and Johnston, {J. M.} and Mendelson, {C. R.}",
year = "1983",
month = "3",
language = "English (US)",
volume = "112",
pages = "1012--1018",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "3",

}

TY - JOUR

T1 - Hormonal regulation of the synthesis of lamellar body phosphatidylglycerol and phosphatidylinositol in fetal lung tissue.

AU - Snyder, J. M.

AU - Longmuir, K. J.

AU - Johnston, J. M.

AU - Mendelson, C. R.

PY - 1983/3

Y1 - 1983/3

N2 - The influence of hormones on the synthesis of phospholipids of lung lamellar bodies was studied using lung tissue explants from 16- to 22-week gestational age human abortuses. Lung explants were incubated in a defined medium containing either no hormonal additions or insulin (2.5 micrograms/ml), cortisol (0.2 microgram/ml), or PRL (2.5 micrograms/ml), added alone or in various combinations. After 7 days of incubation, a purified lamellar body fraction was isolated from the explants. The effects of various hormone combinations on the relative rates of synthesis of lamellar body anionic glycerophospholipids during the last 24 h of culture were analyzed and compared to that of control explants. In control explants, 7.1% of the glycerophospholipid was synthesized as phosphatidylinositol, and 2.2% was synthesized as phosphatidylglycerol. Phosphatidylinositol synthesis was decreased significantly in explants incubated with cortisol alone, insulin plus cortisol, or insulin plus cortisol plus PRL (to 4.4%, 4.3%, and 3.7%, respectively), while phosphatidylglycerol synthesis was increased significantly in lamellar bodies from explants incubated with insulin plus cortisol or insulin plus cortisol plus PRL (to 5.5% and 5.7%, respectively). Although the relative rates of synthesis of lamellar body phosphatidylinositol and phosphatidylglycerol were significantly altered by hormones, the percentages of the total anionic glycerophospholipid were similar in all treatment groups (9.5%). The results of this study, therefore, are suggestive that lamellar body formation and glycerophospholipid composition are under hormonal control.

AB - The influence of hormones on the synthesis of phospholipids of lung lamellar bodies was studied using lung tissue explants from 16- to 22-week gestational age human abortuses. Lung explants were incubated in a defined medium containing either no hormonal additions or insulin (2.5 micrograms/ml), cortisol (0.2 microgram/ml), or PRL (2.5 micrograms/ml), added alone or in various combinations. After 7 days of incubation, a purified lamellar body fraction was isolated from the explants. The effects of various hormone combinations on the relative rates of synthesis of lamellar body anionic glycerophospholipids during the last 24 h of culture were analyzed and compared to that of control explants. In control explants, 7.1% of the glycerophospholipid was synthesized as phosphatidylinositol, and 2.2% was synthesized as phosphatidylglycerol. Phosphatidylinositol synthesis was decreased significantly in explants incubated with cortisol alone, insulin plus cortisol, or insulin plus cortisol plus PRL (to 4.4%, 4.3%, and 3.7%, respectively), while phosphatidylglycerol synthesis was increased significantly in lamellar bodies from explants incubated with insulin plus cortisol or insulin plus cortisol plus PRL (to 5.5% and 5.7%, respectively). Although the relative rates of synthesis of lamellar body phosphatidylinositol and phosphatidylglycerol were significantly altered by hormones, the percentages of the total anionic glycerophospholipid were similar in all treatment groups (9.5%). The results of this study, therefore, are suggestive that lamellar body formation and glycerophospholipid composition are under hormonal control.

UR - http://www.scopus.com/inward/record.url?scp=0020725245&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020725245&partnerID=8YFLogxK

M3 - Article

C2 - 6337040

AN - SCOPUS:0020725245

VL - 112

SP - 1012

EP - 1018

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 3

ER -