Hormone-Sensitive Adenylate Cyclase: Identity, Function, and Regulation of the Protein Components

Elliott M. Ross, Steen E. Pedersen, Vincent A. Florio

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The hormone-sensitive adenylate cyclase system in the plasma membrane of target cells consists of at least three distinct protein species. This chapter discusses the approach to study this hormone-sensitive adenylate cyclase and proteins complex network by trying first to identify and separate these individual protein components, to study them in isolation using increasingly pure preparations, and then to study their interactions and regulation after suitable reconstitution. Results from the laboratory that stress the basic analytical approach to a multienzyme regulatory complex are described. It is assumed that all vertebrate, membrane bound, hormone-sensitive adenylate cyclases are qualitatively similar in their regulation and composition. Observed differences among different cells merely reflect differences in the concentration of each protein or quantitative differences in their kinetic or equilibrium constants rather than qualitative differences in mechanism. As adenylate cyclase components prepared from different cells are shown to be capable of interacting with each other, this assumption is increasingly supported.

Original languageEnglish (US)
Pages (from-to)109-142
Number of pages34
JournalCurrent Topics in Membranes and Transport
Volume18
Issue numberC
DOIs
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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