Abstract
The generation of cell-mediated immunity against many infectious pathogens involves the production of interleukin-12 (IL-12), a key signal of the innate immune system. Yet, for many pathogens, the molecules that induce IL-12 production by macrophages and the mechanisms by which they do so remain undefined. Here it is shown that microbial lipoproteins are potent stimulators of IL-12 production by human macrophages, and that induction is mediated by Toll-like receptors (TLRs). Several lipoproteins stimulated TLR- dependent transcription of inducible nitric oxide synthase and the production of nitric oxide, a powerful microbicidal pathway. Activation of TLRs by microbial lipoproteins may initiate innate defense mechanisms against infectious pathogens.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 732-736 |
| Number of pages | 5 |
| Journal | Science |
| Volume | 285 |
| Issue number | 5428 |
| DOIs | |
| State | Published - Jul 30 1999 |
Fingerprint
ASJC Scopus subject areas
- General
Cite this
Host defense mechanisms triggered by microbial lipoproteins through toll-like receptors. / Brightbill, Hans D.; Libraty, Daniel H.; Krutzik, Stephan R.; Yang, Ruey Bing; Belisle, John T.; Bleharski, Joshua R.; Maitland, Michael; Norgard, Michael V.; Plevy, Scott E.; Smale, Stephen T.; Brennan, Patrick J.; Bloom, Barry R.; Godowski, Paul J.; Modlin, Robert L.
In: Science, Vol. 285, No. 5428, 30.07.1999, p. 732-736.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Host defense mechanisms triggered by microbial lipoproteins through toll-like receptors
AU - Brightbill, Hans D.
AU - Libraty, Daniel H.
AU - Krutzik, Stephan R.
AU - Yang, Ruey Bing
AU - Belisle, John T.
AU - Bleharski, Joshua R.
AU - Maitland, Michael
AU - Norgard, Michael V.
AU - Plevy, Scott E.
AU - Smale, Stephen T.
AU - Brennan, Patrick J.
AU - Bloom, Barry R.
AU - Godowski, Paul J.
AU - Modlin, Robert L.
PY - 1999/7/30
Y1 - 1999/7/30
N2 - The generation of cell-mediated immunity against many infectious pathogens involves the production of interleukin-12 (IL-12), a key signal of the innate immune system. Yet, for many pathogens, the molecules that induce IL-12 production by macrophages and the mechanisms by which they do so remain undefined. Here it is shown that microbial lipoproteins are potent stimulators of IL-12 production by human macrophages, and that induction is mediated by Toll-like receptors (TLRs). Several lipoproteins stimulated TLR- dependent transcription of inducible nitric oxide synthase and the production of nitric oxide, a powerful microbicidal pathway. Activation of TLRs by microbial lipoproteins may initiate innate defense mechanisms against infectious pathogens.
AB - The generation of cell-mediated immunity against many infectious pathogens involves the production of interleukin-12 (IL-12), a key signal of the innate immune system. Yet, for many pathogens, the molecules that induce IL-12 production by macrophages and the mechanisms by which they do so remain undefined. Here it is shown that microbial lipoproteins are potent stimulators of IL-12 production by human macrophages, and that induction is mediated by Toll-like receptors (TLRs). Several lipoproteins stimulated TLR- dependent transcription of inducible nitric oxide synthase and the production of nitric oxide, a powerful microbicidal pathway. Activation of TLRs by microbial lipoproteins may initiate innate defense mechanisms against infectious pathogens.
UR - http://www.scopus.com/inward/record.url?scp=0033618562&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033618562&partnerID=8YFLogxK
U2 - 10.1126/science.285.5428.732
DO - 10.1126/science.285.5428.732
M3 - Article
C2 - 10426995
AN - SCOPUS:0033618562
VL - 285
SP - 732
EP - 736
JO - Science
JF - Science
SN - 0036-8075
IS - 5428
ER -