Host resistance to cyclosporine induced syngeneic graft-versus-host disease. Requirement for two distinct lymphocyte subsets

A. C. Fischer, M. K. Laulis, L. Horwitz, W. E. Beschorner, A. Hess

Research output: Contribution to journalArticle

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Abstract

Cyclosporine is crucial for the prevention of organ allograft rejection and allogeneic graft-vs-host disease (GVHD). Despite its potent immunosuppressive activity, cyclosporine elicits a T cell-mediated autoimmune syndrome after autologous or syngeneic bone marrow transplantation, which has been termed syngeneic GVHD (SGVHD). Recent studies have shown that for disease manifestation, a cytoxan and radiation-sensitive T cell dependent host resistance mechanism must be eliminated, allowing the clonal expansion of autoreactive cells. This report characterizes the autoregulatory lymphocyte population, present in normal animals, capable of inhibiting the adoptive transfer of SGVHD. First, twice the number of unfractionated splenocytes from normal animals to those from autoimmune donors ensured complete inhibition of the adoptive transfer of immune reactivity. Second, the phenotype of this host resistance mechanism in normal splenocytes involves dual regulatory T cell subsets. A helper/inducer subset (W3/25+) must be cotransferred with a cytotoxic/suppressor subset (OX8+) in a ratio that approximates the normal ratio in normal unfractionated splenocytes in order to affect inhibition of the transfer of SGVHD. Moreover the specific inducer regulatory activity resides in the OX22-, W3/25+ subset of Th cells.

Original languageEnglish (US)
Pages (from-to)827-832
Number of pages6
JournalJournal of Immunology
Volume143
Issue number3
StatePublished - 1989

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Adoptive Transfer
Lymphocyte Subsets
Graft vs Host Disease
Cyclosporine
Isogeneic Transplantation
T-Lymphocytes
T-Lymphocyte Subsets
Regulatory T-Lymphocytes
Immunosuppressive Agents
Bone Marrow Transplantation
Cyclophosphamide
Allografts
Lymphocytes
Radiation
Phenotype
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Fischer, A. C., Laulis, M. K., Horwitz, L., Beschorner, W. E., & Hess, A. (1989). Host resistance to cyclosporine induced syngeneic graft-versus-host disease. Requirement for two distinct lymphocyte subsets. Journal of Immunology, 143(3), 827-832.

Host resistance to cyclosporine induced syngeneic graft-versus-host disease. Requirement for two distinct lymphocyte subsets. / Fischer, A. C.; Laulis, M. K.; Horwitz, L.; Beschorner, W. E.; Hess, A.

In: Journal of Immunology, Vol. 143, No. 3, 1989, p. 827-832.

Research output: Contribution to journalArticle

Fischer, AC, Laulis, MK, Horwitz, L, Beschorner, WE & Hess, A 1989, 'Host resistance to cyclosporine induced syngeneic graft-versus-host disease. Requirement for two distinct lymphocyte subsets', Journal of Immunology, vol. 143, no. 3, pp. 827-832.
Fischer, A. C. ; Laulis, M. K. ; Horwitz, L. ; Beschorner, W. E. ; Hess, A. / Host resistance to cyclosporine induced syngeneic graft-versus-host disease. Requirement for two distinct lymphocyte subsets. In: Journal of Immunology. 1989 ; Vol. 143, No. 3. pp. 827-832.
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