Hsp27: A novel therapeutic target for pediatric M4/M5 acute myeloid leukemia

Liangchun Yang, Lizhi Cao, Minghua Yang, Daolin Tang, Rui Kang, Xie Min, Shan Zhu, Yan Yu

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Heat shock protein 27 (Hsp27), a member of the heat shock protein (Hsp) family, is critical in the regulation of cancer development, progression and chemotherapy resistance. However, the role of Hsp27 in the pathogenesis of pediatric acute leukemia (AL) remains unknown. In this study, we evaluated the expression levels of Hsp27 in bone marrow samples from 94 children with newly diagnosed acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML) and 5 leukemia cell lines. Additionally, we transfected a target-specific siRNA duplex against Hsp27 into leukemia cells, and examined the chemosensitivity and cell apoptosis in the response to antitumor drugs. Hsp27 was abundantly expressed in newly diagnosed AML-M4/M5 bone marrow mononuclear cells (BMMCs) and THP-1, OCI/AML-3 leukemia cell lines. Furthermore, its expression was positively correlated with the clinical status in pediatric M4/M5 subtypes. Knockdown of Hsp27 expression increased the chemosensitivity of leukemia cells and the anticancer drug-induced apoptosis. These results support the theory that Hsp27 plays a contributory role in the pathogenesis of pediatric AML-M4/M5. Therefore, Hsp27 may be exploited as a new target for enhancing the efficacy of chemotherapeutic drugs against leukemia.

Original languageEnglish (US)
Pages (from-to)1459-1466
Number of pages8
JournalOncology Reports
Volume29
Issue number4
DOIs
Publication statusPublished - Apr 1 2013
Externally publishedYes

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Keywords

  • Chemosensitivity
  • Heat shock protein 27
  • M4/M5
  • Pediatric acute leukemia

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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