hTR repressor-related gene on human chromosome 10p15.1

N. Miura, N. Onuki, A. Rathi, A. Virmani, S. Nakamoto, Y. Kishimoto, Y. Murawaki, H. Kawasaki, J. Hasegawa, M. Oshimura, W. D. Travis, A. F. Gazdar

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Somatic cells express genes that suppress telomerase activity and these genes may be inactivated in tumour cells. We postulated that cancer cells acquire immortality by activation of telomerase by the loss of such a gene. We have reported recently that a telomerase repressor gene may be located on 10p15.1 by deletion mapping using microcell-mediated chromosome transfer (MMCT), radiated microcell fusion (RMF), fluorescent in situ hybridization (FISH) and STS analysis. To independently confirm this result, we correlated expression of RNA component of telomerase (hTR) as a marker of telomerase expression by in situ hybridization with allelic loss in pulmonary carcinoid tumours. Unlike most malignant tumours, pulmonary carcinoids (which are low-grade malignant tumours) are heterogeneous for telomerase expression. Loss of 5 closely spaced polymorphic markers on 10p15.1, especially D10S1728, were highly correlated with hTR expression. In an additional experiment, 10p15.1 showed higher and more significant correlation than any region of 3p where it has been predicted as another chromosomal location of telomerase repressor with allelic loss of the region. Our findings strongly suggest that 10p15.1 harbours a gene involved in repression of telomerase RNA component in human somatic cells and each putative repressor (on 3p and 10p) may act independently.

Original languageEnglish (US)
Pages (from-to)1510-1514
Number of pages5
JournalBritish journal of cancer
Volume85
Issue number10
DOIs
StatePublished - Nov 16 2001

Keywords

  • Chromosome 10p15
  • In situ hybridization
  • Loss of heterozygosity
  • Mapping
  • Telomerase repressor gene
  • hTR

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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