TY - JOUR
T1 - Human bronchoalveolar lavage fluid protein two-dimensional database
T2 - Study of interstitial lung diseases
AU - Frey, Johann Rudolf
AU - Nguyen, Catherine
AU - Houlgatte, Rémi
AU - Jordan, Bertrand
AU - Auffray, Charles
AU - Fol, Bruno
AU - Kuhn, Lotte
AU - Meyer, Stefan
AU - Hartmann, Klaus Ulrich
AU - Coleclough, Christopher
AU - Kettman, John R.
AU - Lefkovits, Ivan
PY - 2000
Y1 - 2000
N2 - Recently, we published an analytical two-dimensional electrophoresis (2-DE) protein map of human bronchoalveolar lavage fluid (BALF) using a pool of BALFs from various patients. In this report, the effect of lung disorders on the protein composition of the lung epithelial lining fluid was investigated by 2-DE of BALFs from individual patients with well-defined interstitial lung diseases: sarcoidosis, idiopathic pulmonary fibrosis (IPF) and hypersensitivity pneumonitis (HP), using improved experimental conditions. On these gels, about 600-1000 stained protein spots could be identified in a BALF sample containing 25 μg of protein, and our original human BALF protein database has, therefore, been considerably extended. Altogether, 429 protein spots corresponding to 66 different proteins (including isoforms, protein subunits and fragments) were identified by microsequence analysis and by matching with a human blood plasma 2-DE protein map available in the SWISS-2DPAGE database. A human 2-DE BALF database was established and is available on the World Wide Web (http://www.umh.ac.be/≃biochim/proteomic.htm). The significance of the modifications observed between the different lung pathologies is discussed with the aim of understanding the mechanistic bases of lung disease pathogenesis and finding new potential lung markers of disorders.
AB - Recently, we published an analytical two-dimensional electrophoresis (2-DE) protein map of human bronchoalveolar lavage fluid (BALF) using a pool of BALFs from various patients. In this report, the effect of lung disorders on the protein composition of the lung epithelial lining fluid was investigated by 2-DE of BALFs from individual patients with well-defined interstitial lung diseases: sarcoidosis, idiopathic pulmonary fibrosis (IPF) and hypersensitivity pneumonitis (HP), using improved experimental conditions. On these gels, about 600-1000 stained protein spots could be identified in a BALF sample containing 25 μg of protein, and our original human BALF protein database has, therefore, been considerably extended. Altogether, 429 protein spots corresponding to 66 different proteins (including isoforms, protein subunits and fragments) were identified by microsequence analysis and by matching with a human blood plasma 2-DE protein map available in the SWISS-2DPAGE database. A human 2-DE BALF database was established and is available on the World Wide Web (http://www.umh.ac.be/≃biochim/proteomic.htm). The significance of the modifications observed between the different lung pathologies is discussed with the aim of understanding the mechanistic bases of lung disease pathogenesis and finding new potential lung markers of disorders.
KW - Bronchoalveolar lavage
KW - Lung disease
KW - Protein microsequencing
KW - Proteome
KW - Two-dimensional polyacrylamide gel electrophoresis
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U2 - 10.1002/1522-2683(20000701)21:13<2703::AID-ELPS2703>3.0.CO;2-W
DO - 10.1002/1522-2683(20000701)21:13<2703::AID-ELPS2703>3.0.CO;2-W
M3 - Article
C2 - 10949149
AN - SCOPUS:0033846377
SN - 0173-0835
VL - 21
SP - 2703
EP - 2712
JO - ELECTROPHORESIS
JF - ELECTROPHORESIS
IS - 13
ER -