Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis

Zhanyun Tang, Yuxiao Sun, Sara E. Harley, Hui Zou, Hongtao Yu

Research output: Contribution to journalArticle

198 Scopus citations

Abstract

Sister chromatids in mammalian cells remain attached mostly at their centromeres at metaphase because of the loss of cohesion along chromosome arms in prophase. Here, we report that Bub1 retains centromeric cohesion in mitosis of human cells. Depletion of Bub1 or Shugoshin (Sgo1) in HeLa cells by RNA interference causes massive missegregation of sister chromatids that originates at centromeres. Surprisingly, loss of chromatid cohesion in Bub1 and Sgo1 RNA-interference cells does not appear to require the full activation of separase but, instead, triggers a mitotic arrest that depends on Mad2 and Aurora B. Bub1 maintains the steady-state levels and centromeric localization of Sgo1. Therefore, Bub1 protects centromeric cohesion through Shugoshin in mitosis.

Original languageEnglish (US)
Pages (from-to)18012-18017
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number52
DOIs
StatePublished - Dec 28 2004

Keywords

  • Cohesin
  • Kinetochore
  • Spindle checkpoint

ASJC Scopus subject areas

  • General

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