Human calcitonin receptor is directly targeted to and retained in the basolateral surface of MDCK cells

Daniel R. Nussenzveig, Maria De Fatima C Matos, Colette N. Thaw

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The human calcitonin receptor (hCTR) is expressed in polarized cells of the kidney, bone, and nervous system. In the kidney, hCTRs are found in cells of the distal nephron to which blood-borne calcitonin has access only at the basolateral surface. We expressed hCTR subtypes 1 and 2 in Madin-Darby canine kidney (MDCK) cells to establish a cell model useful for delineating the molecular mechanisms underlying hCTR polarity. Selective cell surface incubation demonstrated functional polarity of hCTRs by equilibrium binding or cross-linking of radioiodinated salmon calcitonin (125I-sCT) and cAMP accumulation stimulated by sCT. We estimated that at the steady state there are 40-fold more hCTRs on the basolateral than on the apical side. Domain- selective cell surface biotinylation followed by immunoblotting of streptavidin-agarose-fractionated biotinylated glycoproteins independently confirmed the polarized distribution of FLAG epitope-tagged hCTR-2 in the basolateral domain. Confocal microscopy of immunostained receptors revealed that hCTRs are concentrated on a lateral subdomain of the basolateral membrane. Cell surface arrival assay of newly formed receptors demonstrated that direct delivery to the basolateral domain is the mechanism by which hCTRs become polarized. Measurement of receptor turnover on the basolateral surface showed that retention contributes to hCTR distribution at the steady state.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume275
Issue number5 44-5
StatePublished - 1998

Fingerprint

Calcitonin Receptors
Madin Darby Canine Kidney Cells
salmon calcitonin
Confocal microscopy
Biotinylation
Kidney
Calcitonin
Neurology
Nephrons
Epitopes
Assays
Glycoproteins
Bone
Immunoblotting
Confocal Microscopy
Blood
Nervous System
Membranes
Bone and Bones

Keywords

  • Basolateral sorting
  • G protein- coupled receptor
  • Madin-Darby canine kidney cells
  • Polarized cells
  • Renal epithelial cells

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Human calcitonin receptor is directly targeted to and retained in the basolateral surface of MDCK cells. / Nussenzveig, Daniel R.; Matos, Maria De Fatima C; Thaw, Colette N.

In: American Journal of Physiology - Cell Physiology, Vol. 275, No. 5 44-5, 1998.

Research output: Contribution to journalArticle

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