TY - JOUR
T1 - Human dendritic cell subsets in NOD/SCID mice engrafted with CD34 + hematopoietic progenitors
AU - Palucka, A. Karolina
AU - Gatlin, Joel
AU - Blanck, Jean Philippe
AU - Melkus, Michael W.
AU - Clayton, Sandra
AU - Ueno, Hideki
AU - Kraus, Elizabeth T.
AU - Cravens, Petra
AU - Bennett, Lynda
AU - Padgett-Thomas, Angela
AU - Marches, Florentina
AU - Islas-Ohlmayer, Miguel
AU - Garcia, J. Victor
AU - Banchereau, Jacques
PY - 2003/11/1
Y1 - 2003/11/1
N2 - Distinct human dendritic cell (DC) subsets differentially control immunity. Thus, insights into their in vivo functions are important to understand the launching and modulation of immune responses. We show that nonobese diabetic/LtSz-scid/scid (NOD/SCID) mice engrafted with human CD34+ hematopoietic progenitors develop human myeloid and plasmacytoid DCs. The skin displays immature DCs expressing Langerin, while other tissues display interstitial DCs. Myeloid DCs from these mice induce proliferation of allogeneic CD4 T cells in vitro, and bone marrow human cells containing plasmacytoid DCs release interferon-α (IFN-α) upon influenza virus exposure. Injection of influenza virus into reconstituted mice triggers IFN-α release and maturation of mDCs. Thus, these mice may provide a model to study the pathophysiology of human DC subsets.
AB - Distinct human dendritic cell (DC) subsets differentially control immunity. Thus, insights into their in vivo functions are important to understand the launching and modulation of immune responses. We show that nonobese diabetic/LtSz-scid/scid (NOD/SCID) mice engrafted with human CD34+ hematopoietic progenitors develop human myeloid and plasmacytoid DCs. The skin displays immature DCs expressing Langerin, while other tissues display interstitial DCs. Myeloid DCs from these mice induce proliferation of allogeneic CD4 T cells in vitro, and bone marrow human cells containing plasmacytoid DCs release interferon-α (IFN-α) upon influenza virus exposure. Injection of influenza virus into reconstituted mice triggers IFN-α release and maturation of mDCs. Thus, these mice may provide a model to study the pathophysiology of human DC subsets.
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U2 - 10.1182/blood-2003-02-0384
DO - 10.1182/blood-2003-02-0384
M3 - Article
C2 - 12869510
AN - SCOPUS:0142214633
SN - 0006-4971
VL - 102
SP - 3302
EP - 3310
JO - Blood
JF - Blood
IS - 9
ER -