Human fetal liver estrogen 16α-Hydroxylase: Precursor specificity, kinetic parameters, and in vitro regulation

L. Milewich, P. C. MacDonald, A. Guerami, W. T. Midgett, W. L. Lassiter, B. R. Carr

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Abstract

The properties of human fetal liver (HFL) estrogen 16α-hydroxylase (16α-OHase) were studied in microsomal preparations and hepatocytes maintained in culture. A specific assay was developed for the determination of estrogen 16α- OHase activity based on the enzymatic release of tritium from the C-16α position of stereospecifically labeled 16α-3H-labeled C18-steroids, viz. 17β-[16α-3H]estradiol, 17βl6α-3H]estradiol3- sulfate, [16α-3H]estrone, and [16α-3H]estrone sulfate. The percentage of tritium at the C-16α position of 17β[16α-3H]estradiol was 92.5%. There was no kinetic isotope effect in the 16αhydroxylation of 17β[16α-3H]estradiol. HFL hepatocyte and microsomal 16α-OHase activity was linear with incubation time for at least 2 h, with a hepatocyte number up to 6.7 × 106 cells/ml and a microsomal protein concentration up to 1 mg/ml. The apparent Km of 16α-OHase for estrone sulfate (E1S) was greater than that for either 17β-estradiol (E2) or estrone (El; 2.9-6.4 μM us. 0.70-0.84 μM). The maximum velocity of HFL 16α-OHase also was greater with ElS or 17β-estradiol 3-sulfate than with either El or E2, and ElS was the most efficient substrate. The apparent temperature optimum for the microsomal enzyme was 37 C, and the apparent pH optimum was 7.0.16α-Hydroxylation of ElS by HFL microsomes was inhibited noncompetitively by El. The biosynthesis of estriol from E2 by fetal liver microsomes does not require an intermediate oxidation step of E2 to El as appears to be the case in vivo in the human adult; this was demonstrated by the formation of [17α-3H]estriol from [17α-3H] E2 in incubations with HFL microsomes. A number of growth factors, hormones, and xenobiotics were preincubated with hepatocytes for 24 or 72 h to test for stimulation/inhibition of 16α-OHase activity. 16α-OHase activity was stimulated by dexamethasone, forskolin, (Bu)2cAMP, cholera toxin, 1,2-benzanthracene, and phenobarbital. Diethylstilbestrol, E2, and progesterone did not alter hepatocyte 16α-OHase activity, except when very high concentrations of E2 and progesterone were used, when they became inhibitory; other hormones and growth factors did not alter the basal levels of the enzyme.

Original languageEnglish (US)
Pages (from-to)180-191
Number of pages12
JournalJournal of Clinical Endocrinology and Metabolism
Volume63
Issue number1
DOIs
StatePublished - Jul 1986

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ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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