Human heat shock protein 70 (hsp70) protects murine cells from injury during metabolic stress

R. Sanders Williams, James A. Thomas, Maggy Fina, Zohre German, Ivor J. Benjamin

Research output: Contribution to journalArticle

150 Scopus citations

Abstract

Expression of heat shock protein 70 (hsp70) is stimulated during ischemia, but its proposed cytoprotective function during metabolic stress has remained conjectural. We introduced a human hsp70 gene into mouse 10T 1/2 cells and assessed the susceptibility of these cells to injury in response to conditions that mimic ischemia. Transiently transfected cells, in the absence of stress, expressed human hsp70 to levels equal to or greater than those induced by heat shock, as assessed by RNAse protection, immunoblot, and immunohistochemical analyses. By comparison to cells transfected with a control plasmid, cells expressing the human hsp70 transgene were resistant to injury induced by glucose deprivation and inhibition of mitochondrial respiration. These results provide direct evidence for a cytoprotective function of hsp70 during metabolic stress.

Original languageEnglish (US)
Pages (from-to)503-508
Number of pages6
JournalJournal of Clinical Investigation
Volume92
Issue number1
DOIs
StatePublished - 1993

Keywords

  • cytoprotection
  • heat shock protein 70
  • ischemia
  • stress proteins

ASJC Scopus subject areas

  • Medicine(all)

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