Germfree rats transgenic for the human genes HLA-B27 and β2- microglobulin were colonized with hemolysin-positive (Hly+) or hemolysin- negative (Hly-) strains of Listeria monocytogenes. HLA-B27 rats were very susceptible to infection with Hly+ L. monocytogenes; none survived beyond 6 days. Conversely, nontransgenic control rats survived alimentary tract colonization with the Hly+ strain, and both transgenic and nontransgenic rats survived colonization with the Hly- strain of L. monocytogenes. After colonization with Hly+ L. monocytogenes, both transgenic and nontransgenic rats developed severe bowel inflammation, which consisted histologically of microabscesses, granulomatous lesions, and ulcers; however, whereas the transgenic rats died within 6 days, only very mild intestinal lesions were seen in nontransgenic rats 10 to 42 days after colonization. Liver and splenic lesions were small and transient in nontransgenic rats. Transgenic and nontransgenic control rats infected with Hly- Listeria developed mild transient diarrhea but showed no histological changes in the intestine. This study thus documents an association between a particular bacterial product (hemolysin produced by L. monocytogenes) and the induction of severe inflammatory disease and death in rats expressing HLA-B27 and β2- microglobulin.
|Original language||English (US)|
|Number of pages||7|
|Journal||American Journal of Pathology|
|State||Published - Nov 1996|
ASJC Scopus subject areas
- Pathology and Forensic Medicine