Human intestinal parasite burden and poor sanitation in rural Alabama

Megan L. McKenna, Shannon McAtee, Patricia E. Bryan, Rebecca Jeun, Tabitha Ward, Jacob Kraus, Maria E. Bottazzi, Peter J. Hotez, Catherine C. Flowers, Rojelio Mejia

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Hookworm infection affects 430 million people worldwide, causing iron deficiency, impaired cognitive development, and stunting in children. Because of the environmental conditions needed for the hookworm life-cycle, this parasite is endemic to resource-limited countries. Necator americanus was endemic in the southern United States before improvement of sewage disposal systems and eradication programs. With continued poverty, poor sanitation, and an environment suitable for the hookworm life-cycle in some regions of the southern United States, a current prevalence study using modern molecular diagnostics is warranted. Lowndes County, Alabama, was chosen as the study site given previous high hookworm burdens, degree of poverty, and use of open-sewage systems. Participants were interviewed, and stool, serum, and soil samples were tested for nine intestinal parasites using a multiparallel quantitative real-time PCR (qPCR) and enzyme-linked immunosorbent assays. We found that, among 24 households, 42.4% reported exposure to raw sewage within their home, and from 55 stool samples, 19 (34.5%) tested positive for N. americanus, four (7.3%) for Strongyloides stercoralis, and one (1.8%) for Entamoeba histolytica. Stool tested positive for N. americanus contained low levels of parasite DNA (geometric mean 0.0302 fg/μL). Soil studies detected one (2.9%) Cryptosporidium species, and Toxocara serology assay detected one (5.2%) positive in this population. Individuals living in this high-risk environment within the United States continue to have stool samples positive for N. americanus. Gastrointestinal parasites known to be endemic to developing countries are identifiable in American poverty regions, and areas with lower disease burden are more likely to be identified by using qPCR.

Original languageEnglish (US)
Pages (from-to)1623-1628
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume97
Issue number5
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Fingerprint

Necator americanus
Sanitation
Ancylostomatoidea
Parasites
Sewage
Poverty
Life Cycle Stages
Soil
Hookworm Infections
Toxocara
Growth Disorders
Strongyloides stercoralis
Poverty Areas
Cryptosporidium
Entamoeba histolytica
Molecular Pathology
Serology
Developing Countries
Real-Time Polymerase Chain Reaction
Iron

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

Cite this

Human intestinal parasite burden and poor sanitation in rural Alabama. / McKenna, Megan L.; McAtee, Shannon; Bryan, Patricia E.; Jeun, Rebecca; Ward, Tabitha; Kraus, Jacob; Bottazzi, Maria E.; Hotez, Peter J.; Flowers, Catherine C.; Mejia, Rojelio.

In: American Journal of Tropical Medicine and Hygiene, Vol. 97, No. 5, 01.01.2017, p. 1623-1628.

Research output: Contribution to journalArticle

McKenna, ML, McAtee, S, Bryan, PE, Jeun, R, Ward, T, Kraus, J, Bottazzi, ME, Hotez, PJ, Flowers, CC & Mejia, R 2017, 'Human intestinal parasite burden and poor sanitation in rural Alabama', American Journal of Tropical Medicine and Hygiene, vol. 97, no. 5, pp. 1623-1628. https://doi.org/10.4269/ajtmh.17-0396
McKenna, Megan L. ; McAtee, Shannon ; Bryan, Patricia E. ; Jeun, Rebecca ; Ward, Tabitha ; Kraus, Jacob ; Bottazzi, Maria E. ; Hotez, Peter J. ; Flowers, Catherine C. ; Mejia, Rojelio. / Human intestinal parasite burden and poor sanitation in rural Alabama. In: American Journal of Tropical Medicine and Hygiene. 2017 ; Vol. 97, No. 5. pp. 1623-1628.
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