Human melanoma cells express functional endothelin-1 receptors

J. J. Yohn, C. Smith, T. Stevens, T. A. Hoffman, J. G. Morelli, D. L. Hurt, Masashi Yanagisawa, M. A. Kane, M. R. Zamora

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


Current evidence suggests that endothelium-derived factors enhance human melanoma vascular invasion. Therefore, we studied human melanoma cell expression of receptors to the endothelium-derived peptide, endothelin-1 (ET-1), and determined if they respond to ET-1 with proliferation and chemokinesis. Human metastatic melanoma cell lines were found to have specific, saturable, high affinity ET-1 binding. Northern analysis and competitive inhibition studies confirmed that melanoma cells express the ETB receptor isoform. Ten nanomolar ET-1 caused an 8.2 to 25.5-fold increase in intracellular free calcium. ET-1 was found to be a weak mitogen for melanoma cells, however, melanoma cell chemokinesis was significantly increased by ET-1. These data suggest that ET-1 may be involved in providing a chemokinetic and growth factor environment that enhances perivascular proliferation and invasiveness of melanoma cells.

Original languageEnglish (US)
Pages (from-to)449-457
Number of pages9
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - May 30 1994

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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