Human Melanoma Cells Express Functional Endothelin-1 Receptors

J. J. Yohn, C. Smith, T. Stevens, T. A. Hoffman, J. G. Morelli, D. L. Hurt, Masashi Yanagisawa, M. A. Kane, M. R. Zamora

Research output: Contribution to journalArticle

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Abstract

Current evidence suggests that endothelium-derived factors enhance human melanoma vascular invasion. Therefore, we studied human melanoma cell expression of receptors to the endothelium-derived peptide, endothelin-1 (ET-1), and determined if they respond to ET-1 with proliferation and chemokinesis. Human metastatic melanoma cell lines were found to have specific, saturable, high affinity ET-1 binding. Northern analysis and competitive inhibition studies confirmed that melanoma cells express the ETB receptor isoform. Ten nanomolar ET-1 caused an 8.2 to 25.5-fold increase in intracellular free calcium. ET-1 was found to be a weak mitogen for melanoma cells, however, melanoma cell chemokinesis was significantly increased by ET-1. These data suggest that ET-1 may be involved in providing a chemokinetic and growth factor environment that enhances perivascular proliferation and invasiveness of melanoma cells.

Original languageEnglish (US)
Pages (from-to)449-457
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume201
Issue number1
DOIs
StatePublished - May 30 1994

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Endothelin A Receptors
Endothelin-1
Melanoma
Endothelium
Human engineering
Mitogens
Blood Vessels
Intercellular Signaling Peptides and Proteins
Protein Isoforms
Cells
Calcium
Cell Line
Peptides

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Yohn, J. J., Smith, C., Stevens, T., Hoffman, T. A., Morelli, J. G., Hurt, D. L., ... Zamora, M. R. (1994). Human Melanoma Cells Express Functional Endothelin-1 Receptors. Biochemical and Biophysical Research Communications, 201(1), 449-457. https://doi.org/10.1006/bbrc.1994.1722

Human Melanoma Cells Express Functional Endothelin-1 Receptors. / Yohn, J. J.; Smith, C.; Stevens, T.; Hoffman, T. A.; Morelli, J. G.; Hurt, D. L.; Yanagisawa, Masashi; Kane, M. A.; Zamora, M. R.

In: Biochemical and Biophysical Research Communications, Vol. 201, No. 1, 30.05.1994, p. 449-457.

Research output: Contribution to journalArticle

Yohn, JJ, Smith, C, Stevens, T, Hoffman, TA, Morelli, JG, Hurt, DL, Yanagisawa, M, Kane, MA & Zamora, MR 1994, 'Human Melanoma Cells Express Functional Endothelin-1 Receptors', Biochemical and Biophysical Research Communications, vol. 201, no. 1, pp. 449-457. https://doi.org/10.1006/bbrc.1994.1722
Yohn, J. J. ; Smith, C. ; Stevens, T. ; Hoffman, T. A. ; Morelli, J. G. ; Hurt, D. L. ; Yanagisawa, Masashi ; Kane, M. A. ; Zamora, M. R. / Human Melanoma Cells Express Functional Endothelin-1 Receptors. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 201, No. 1. pp. 449-457.
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