Human memory T cell differentiation into Th2-like effector cells is dependent on IL-4 and CD28 stimulation and inhibited by TCR ligation

Hendrik Schulze-Koops, Peter E. Lipsky, Laurie S. Davis

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Freshly isolated memory T cells primarily produced IL-2 and small amounts of IL-4 and IFN-γ after stimulation in vitro. Priming for 5 days in vitro with anti-CD28 monoclonal antibodies (mAb) alone markedly increased production of IL-4. In comparison to fresh cells, the increase in the amount of IL-4 secreted reflected a marked increase in the number of IL-4-producing cells. Stimulation with immobilized anti-CD3 mAb during priming limited subsequent IL-4 production. By contrast, IFN-γ production from in vitro primed memory T cells was directly correlated to the concentration of priming anti-CD3 mAb. IL-2 production by all restimulated cells was decreased. The differentiation of IL-4-producing cells could be blocked by antibody to IL-4 and enhanced by the addition of recombinant IL-4 as well as antibody to IFN-γ. Of note, the IL-4-producing effector cells induced from in vitro priming derived from the early CD27(pos) memory T cell subset, whereas the small CD27(neg) differentiated memory subset produced IL-4 without in vitro priming. The results indicate that memory T cells can be directed to differentiate into IL-4-producing effector cells by stimulation via CD28 and IL-4, whereas increasing engagement of the TCR limits Th2 memory cell differentiation.

Original languageEnglish (US)
Pages (from-to)2517-2529
Number of pages13
JournalEuropean Journal of Immunology
Volume28
Issue number8
DOIs
StatePublished - Aug 1 1998

Keywords

  • Cytokine
  • Human
  • Memory
  • Th2

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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