Human natural killer cells: A unique innate immunoregulatory role for the CD56BRIGHT SUBSET

Megan A. Cooper, Todd A. Fehniger, Sarah C. Turner, Kenneth S. Chen, Bobak A. Ghaheri, William E. Carson, Michael A. Caligiuri

Research output: Contribution to journalArticle

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Abstract

Human natural killer (NK) cells produce immunoregulatory cytokines in response to stimulation with monocyte-derived cytokines (monokines). These NK-derived cytokines (e.g. IFN-γ, GM-CSF) are important for the host's early defense against a variety of bacterial, viral, and parasitic pathogens. Human NK cell subsets can be distinguished by CD56 surface density expression (e.g. CD56bright and CD56dim). In this study we have investigated cytokine production by NK cell subsets in response to stimulation with recombinant monokines or phorbol esters (PMA) and ionomycin. CD56bright NK cells produced significantly greater levels of IFN-γ, TNF-β, GM-CSF, TNF-α, IL-10, and IL13 protein than CD56dim NK cells. Furthermore, CD56bright NK cells co-cultured with LPS-activated macrophages (an endogenous source of monokines) produced significantly more cytokines than the CD56dlm NK cell subset. Therefore, we present novel evidence showing that CD56bright NK cells are the primary source of NK cell-derived immunoregulatory cytokines. These data support a model whereby CD56bright and CD56dim NK cells represent functionally distinct subsets of mature human NK cells. Knowledge of the differential functional attributes of CD56bright (e.g. immunoregulatory cytokine production) and CD56dim (e.g. cytotoxicity) subsets may enable us to design strategies that preferentially activate that subset with the greatest therapeutic potential for a particular disease.

Original languageEnglish (US)
JournalBlood
Volume96
Issue number11 PART II
StatePublished - Dec 1 2000

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Natural Killer Cells
Cytokines
Monocytes
Granulocyte-Macrophage Colony-Stimulating Factor
Ionomycin
Interleukin-13
Macrophages
Phorbol Esters
Pathogens
Cytotoxicity
Interleukin-10

ASJC Scopus subject areas

  • Hematology

Cite this

Cooper, M. A., Fehniger, T. A., Turner, S. C., Chen, K. S., Ghaheri, B. A., Carson, W. E., & Caligiuri, M. A. (2000). Human natural killer cells: A unique innate immunoregulatory role for the CD56BRIGHT SUBSET. Blood, 96(11 PART II).

Human natural killer cells : A unique innate immunoregulatory role for the CD56BRIGHT SUBSET. / Cooper, Megan A.; Fehniger, Todd A.; Turner, Sarah C.; Chen, Kenneth S.; Ghaheri, Bobak A.; Carson, William E.; Caligiuri, Michael A.

In: Blood, Vol. 96, No. 11 PART II, 01.12.2000.

Research output: Contribution to journalArticle

Cooper, MA, Fehniger, TA, Turner, SC, Chen, KS, Ghaheri, BA, Carson, WE & Caligiuri, MA 2000, 'Human natural killer cells: A unique innate immunoregulatory role for the CD56BRIGHT SUBSET', Blood, vol. 96, no. 11 PART II.
Cooper MA, Fehniger TA, Turner SC, Chen KS, Ghaheri BA, Carson WE et al. Human natural killer cells: A unique innate immunoregulatory role for the CD56BRIGHT SUBSET. Blood. 2000 Dec 1;96(11 PART II).
Cooper, Megan A. ; Fehniger, Todd A. ; Turner, Sarah C. ; Chen, Kenneth S. ; Ghaheri, Bobak A. ; Carson, William E. ; Caligiuri, Michael A. / Human natural killer cells : A unique innate immunoregulatory role for the CD56BRIGHT SUBSET. In: Blood. 2000 ; Vol. 96, No. 11 PART II.
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