Human papillomavirus-16 E6/E7 transfected retinal cell line expresses the Muller cell phenotype

Rouel S. Roque, Neeraj Agarwal, Robert J. Wordinger, Anne Marie Brun, Yaming Xue, Lan C. Huang, Loc P. Nguyen, Jerry W. Shay

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

The introduction of viral transforming genes into mammalian cells has been used in establishing cultures of unlimited lifespan. Although Muller cells, the predominant glial cells in the mammalian retina, have been isolated using a variety of techniques, most of these cultures have limited capacity for cell division and are often contaminated by other cell types especially astrocytes, endothelial cells and microglial cells. We have established pure cultures of retinal cells which express Muller cell characteristics and exhibit unlimited growth in vitro. We now report the techniques involved in the propagation and characterization of these cultures. Mixed retinal cultures isolated from dystrophic rat retinas were infected with defective retroviruses coding for human papillomavirus (HPV) type 16 E6 and E7 proteins. The disabled viral constructs also contained the neomycin gene allowing selection of the cultures using Geneticin, a neomycin analogue. Pure cultures were then obtained from Geneticin-selected populations by limiting end-dilution techniques. The expression of the HPV- 16 E6/E7 genes in the transfected cell line was established using an HPV-16 E6/E7 PCR product to probe Northern blots. Cloned cells were found to be highly reactive for Muller cell markers including S-100, carbonic anhydrase- C, cellular retinaldehyde binding protein, and glial fibrillary acidic protein but not for glutamine synthetase. Ultrastructural studies showed stacks of cells with long elaborate processes, short microvilli, coated pits, cytoplasmic filaments, abundant perinuclear rough endoplasmic reticulum, and smooth endoplasmic reticulum extending to the cell processes. Growth patterns of late passage cells (> 50 passages) showed a lag phase of 48 hr followed by exponential growth extending past visual confluence at day 5. Since the cultures have undergone more than 240 population doublings, they can be characterized as a continuous cell line with unlimited lifespan. The HPV-16 E6/E7 transfected Muller cell line may prove useful in studies requiring abundant and pure cultures of Muller cells.

Original languageEnglish (US)
Pages (from-to)519-527
Number of pages9
JournalExperimental Eye Research
Volume64
Issue number4
DOIs
StatePublished - Apr 1997

Keywords

  • Carbonic anhydrase-C
  • Cellular retinaldehyde binding protein
  • Dystrophic retina
  • Glial fibrillary acidic protein
  • Glutamine synthetase
  • Human papilloma virus-16 E6 and E7
  • Immunoblotting
  • Immunocytochemistry
  • Microglial cells
  • Northern blotting
  • Retinal glia
  • S-100
  • Viral transfection

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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  • Cite this

    Roque, R. S., Agarwal, N., Wordinger, R. J., Brun, A. M., Xue, Y., Huang, L. C., Nguyen, L. P., & Shay, J. W. (1997). Human papillomavirus-16 E6/E7 transfected retinal cell line expresses the Muller cell phenotype. Experimental Eye Research, 64(4), 519-527. https://doi.org/10.1006/exer.1996.0230