Human paraoxonases (PON1, PON2, and PON3) are lactonases with overlapping and distinct substrate specificities

Dragomir I. Draganov, John F. Teiber, Audrey Speelman, Yoichi Osawa, Roger Sunahara, Bert N. La Du

Research output: Contribution to journalArticle

459 Citations (Scopus)

Abstract

The paraoxonase (PON) gene family in humans has three members, PON1, PON2, and PON3. Their physiological role(s) and natural substrates are uncertain. We developed a baculovirus-mediated expression system, suitable for all three human PONs, and optimized procedures for their purification. The recombinant PONs are glycosylated with high-mannose-type sugars, which are important for protein stability but are not essential for their enzymatic activities. Enzymatic characterization of the purified PONs has revealed them to be lactonases/lactonizing enzymes, with some overlapping substrates (e.g., aromatic lactones), but also to have distinctive substrate specificities. All three PONs metabolized very efficiently 5-hydroxy-eicosatetraenoic acid 1,5-lactone and 4-hydroxy-docosahexaenoic acid, which are products of both enzymatic and nonenzymatic oxidation of arachidonic acid and docosahexaenoic acid, respectively, and may represent the PONs' endogenous substrates. Organophosphates are hydrolyzed almost exclusively by PON1, whereas bulky drug substrates such as lovastatin and spironolactone are hydrolyzed only by PON3. Of special interest is the ability of the human PONs, especially PON2, to hydrolyze and thereby inactivate N-acyl-homoserine lactones, which are quorum-sensing signals of pathogenic bacteria. None of the recombinant PONs protected low density lipoprotein against copper-induced oxidation in vitro.

Original languageEnglish (US)
Pages (from-to)1239-1247
Number of pages9
JournalJournal of Lipid Research
Volume46
Issue number6
DOIs
StatePublished - 2005

Fingerprint

Substrate Specificity
Hydroxy Acids
Docosahexaenoic Acids
Lactones
Substrates
Acyl-Butyrolactones
Arachidonic Acids
Aryldialkylphosphatase
Lovastatin
Quorum Sensing
Spironolactone
Organophosphates
Protein Stability
Baculoviridae
Mannose
LDL Lipoproteins
Arachidonic Acid
Copper
Oxidation
Bacteria

Keywords

  • Arylesterase
  • Low density lipoprotein oxidation
  • N-acyl-homoserine lactones
  • Protein expression and purification

ASJC Scopus subject areas

  • Endocrinology

Cite this

Human paraoxonases (PON1, PON2, and PON3) are lactonases with overlapping and distinct substrate specificities. / Draganov, Dragomir I.; Teiber, John F.; Speelman, Audrey; Osawa, Yoichi; Sunahara, Roger; La Du, Bert N.

In: Journal of Lipid Research, Vol. 46, No. 6, 2005, p. 1239-1247.

Research output: Contribution to journalArticle

Draganov, Dragomir I. ; Teiber, John F. ; Speelman, Audrey ; Osawa, Yoichi ; Sunahara, Roger ; La Du, Bert N. / Human paraoxonases (PON1, PON2, and PON3) are lactonases with overlapping and distinct substrate specificities. In: Journal of Lipid Research. 2005 ; Vol. 46, No. 6. pp. 1239-1247.
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AU - Sunahara, Roger

AU - La Du, Bert N.

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