Human periosteum cell osteogenic differentiation enhanced by ionic silicon release from porous amorphous silica fibrous scaffolds

Tetsurou Odatsu, Taha Azimaie, Megan F. Velten, Michael Vu, Mark B. Lyles, Harry K. Kim, Pranesh B. Aswath, Venu G. Varanasi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Current synthetic grafts for bone defect filling in the sinus can support new bone formation but lack the ability to stimulate or enhance osteogenic healing. To promote such healing, osteoblast progenitors such as human periosteum cells must undergo osteogenic differentiation. In this study, we tested the hypothesis that degradation of porous amorphous silica fibrous (PASF) scaffolds can enhance human periosteum cell osteogenic differentiation. Two types of PASF were prepared and evaluated according to their densities (PASF99, PASF98) with 99 and 98% porosity, respectively. Silicon (Si) ions were observed to rapidly release from both scaffolds within 24 h in vitro. PASF99 Si ion release rate was estimated to be nearly double that of PASF98 scaffolds. Mechanical tests revealed a lower compressive strength in PASF99 as compared with PASF98. Osteogenic expression analysis showed that PASF99 scaffolds enhanced the expression of activating transcription factor 4, alkaline phosphatase, and collagen (Col(I)α1, Col(I)α2). Scanning electron microscopy showed cellular and extracellular matrix (ECM) ingress into both scaffolds within 16 days and the formation of Ca-P precipitates within 85 days. In conclusion, this study demonstrated that PASF scaffolds enhance human periosteum cell osteogenic differentiation by releasing ionic Si, and structurally supporting cellular and ECM ingress.

Original languageEnglish (US)
Pages (from-to)2797-2806
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume103
Issue number8
DOIs
StatePublished - Aug 1 2015

Fingerprint

Silicon
Scaffolds
Silicon Dioxide
Silica
Bone
Activating Transcription Factor 4
Transcription factors
Ions
Osteoblasts
Phosphatases
Scaffolds (biology)
Collagen
Grafts
Compressive strength
Precipitates
Porosity
Alkaline Phosphatase
Degradation
Defects
Scanning electron microscopy

Keywords

  • osteocalcin
  • periosteum cell
  • Runx2
  • scaffold
  • Si

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

Cite this

Human periosteum cell osteogenic differentiation enhanced by ionic silicon release from porous amorphous silica fibrous scaffolds. / Odatsu, Tetsurou; Azimaie, Taha; Velten, Megan F.; Vu, Michael; Lyles, Mark B.; Kim, Harry K.; Aswath, Pranesh B.; Varanasi, Venu G.

In: Journal of Biomedical Materials Research - Part A, Vol. 103, No. 8, 01.08.2015, p. 2797-2806.

Research output: Contribution to journalArticle

Odatsu, Tetsurou ; Azimaie, Taha ; Velten, Megan F. ; Vu, Michael ; Lyles, Mark B. ; Kim, Harry K. ; Aswath, Pranesh B. ; Varanasi, Venu G. / Human periosteum cell osteogenic differentiation enhanced by ionic silicon release from porous amorphous silica fibrous scaffolds. In: Journal of Biomedical Materials Research - Part A. 2015 ; Vol. 103, No. 8. pp. 2797-2806.
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