Human polymorphonuclear leukocyte phagocytosis of crystalline cholesterol, bilirubin, and calcium hydroxyapatite in vitro

Jay B. Prystowsky, Jayashree S. Huprikar, Alfred W. Rademaker, Robert V Rege

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

We tested the hypothesis that human polymorphonuclear leukocytes (PMNs) phagocytize crystalline cholesterol, bilirubin, or calcium hydroxyapatite in vitro and in the process release oxygen metabolites and enzymes involved in the inflammatory process. Chemiluminescence (CL), elicited by the respiratory burst (release and activation of oxygen metabolites and enzymes) of PMNs during phagocytosis of a target particle, was used to quantitate PMN phagocytosis of each crystal. Significant CL (P<0.05) was observed with cholesterol concentrations of 1.3-5.3 mg/ml and the dose-response was linear (r≥0.95). With bilirubin, significant CL was observed with concentrations of 0.07-0.33 mg/ml. The response to calcium hydroxyapatite was variable. Human PMNs phagocytize cholesterol, bilirubin, and to a lesser extent, calcium hydroxyapatite. PMN chemiluminescence was associated with phagocytosis, indicating that inflammatory substances are being released in the process. These results support the concept that crystals that occur in the gallbladder may initiate gallbladder inflammation.

Original languageEnglish (US)
Pages (from-to)412-418
Number of pages7
JournalDigestive Diseases and Sciences
Volume40
Issue number2
DOIs
StatePublished - Feb 1 1995

Keywords

  • crystals
  • gallbladder
  • phagocytosis
  • polymorphonuclear leukocytes

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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