Human postmortem tissue

What quality markers matter?

Ana D. Stan, Subroto Ghose, Xue Min Gao, Rosalinda C. Roberts, Kelly Lewis-Amezcua, Kimmo J. Hatanpaa, Carol A. Tamminga

Research output: Contribution to journalArticle

223 Citations (Scopus)

Abstract

Postmortem human brain tissue is used for the study of many different brain diseases. A key factor in conducting postmortem research is the quality of the tissue. Unlike animal tissue, whose condition at death can be controlled and influenced, human tissue can only be collected naturalistically. This introduces potential confounds, based both on pre- and postmortem conditions, that may influence the quality of tissue and its ability to yield accurate results. The traditionally recognized confounds that reduce tissue quality are agonal factors (e.g., coma, hypoxia, hyperpyrexia at the time of death), and long postmortem interval (PMI). We measured tissue quality parameters in over 100 postmortem cases collected from different sources and correlated them with RNA quality (as indicated by the RNA Integrity Number (RIN)) and with protein quality (as measured by the level of representative proteins). Our results show that the most sensible indicator of tissue quality is RIN and that there is a good correlation between RIN and the pH. No correlation developed between protein levels and the aforementioned factors. Moreover, even when RNA was degraded, the protein levels remained stable. However, these correlations did not prove true under all circumstances (e.g., thawed tissue, surgical tissue), that yielded unexpected quality indicators. These data also suggest that cases whose source was a Medical Examiner's office represent high tissue quality.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalBrain Research
Volume1123
Issue number1
DOIs
StatePublished - Dec 6 2006

Fingerprint

RNA
Proteins
Coroners and Medical Examiners
Brain Diseases
Coma
Brain
Research

Keywords

  • Brain
  • pH
  • PMI
  • RIN

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Human postmortem tissue : What quality markers matter? / Stan, Ana D.; Ghose, Subroto; Gao, Xue Min; Roberts, Rosalinda C.; Lewis-Amezcua, Kelly; Hatanpaa, Kimmo J.; Tamminga, Carol A.

In: Brain Research, Vol. 1123, No. 1, 06.12.2006, p. 1-11.

Research output: Contribution to journalArticle

Stan, Ana D. ; Ghose, Subroto ; Gao, Xue Min ; Roberts, Rosalinda C. ; Lewis-Amezcua, Kelly ; Hatanpaa, Kimmo J. ; Tamminga, Carol A. / Human postmortem tissue : What quality markers matter?. In: Brain Research. 2006 ; Vol. 1123, No. 1. pp. 1-11.
@article{5dbf26bef73c46b0bc1b20a782487e13,
title = "Human postmortem tissue: What quality markers matter?",
abstract = "Postmortem human brain tissue is used for the study of many different brain diseases. A key factor in conducting postmortem research is the quality of the tissue. Unlike animal tissue, whose condition at death can be controlled and influenced, human tissue can only be collected naturalistically. This introduces potential confounds, based both on pre- and postmortem conditions, that may influence the quality of tissue and its ability to yield accurate results. The traditionally recognized confounds that reduce tissue quality are agonal factors (e.g., coma, hypoxia, hyperpyrexia at the time of death), and long postmortem interval (PMI). We measured tissue quality parameters in over 100 postmortem cases collected from different sources and correlated them with RNA quality (as indicated by the RNA Integrity Number (RIN)) and with protein quality (as measured by the level of representative proteins). Our results show that the most sensible indicator of tissue quality is RIN and that there is a good correlation between RIN and the pH. No correlation developed between protein levels and the aforementioned factors. Moreover, even when RNA was degraded, the protein levels remained stable. However, these correlations did not prove true under all circumstances (e.g., thawed tissue, surgical tissue), that yielded unexpected quality indicators. These data also suggest that cases whose source was a Medical Examiner's office represent high tissue quality.",
keywords = "Brain, pH, PMI, RIN",
author = "Stan, {Ana D.} and Subroto Ghose and Gao, {Xue Min} and Roberts, {Rosalinda C.} and Kelly Lewis-Amezcua and Hatanpaa, {Kimmo J.} and Tamminga, {Carol A.}",
year = "2006",
month = "12",
day = "6",
doi = "10.1016/j.brainres.2006.09.025",
language = "English (US)",
volume = "1123",
pages = "1--11",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Human postmortem tissue

T2 - What quality markers matter?

AU - Stan, Ana D.

AU - Ghose, Subroto

AU - Gao, Xue Min

AU - Roberts, Rosalinda C.

AU - Lewis-Amezcua, Kelly

AU - Hatanpaa, Kimmo J.

AU - Tamminga, Carol A.

PY - 2006/12/6

Y1 - 2006/12/6

N2 - Postmortem human brain tissue is used for the study of many different brain diseases. A key factor in conducting postmortem research is the quality of the tissue. Unlike animal tissue, whose condition at death can be controlled and influenced, human tissue can only be collected naturalistically. This introduces potential confounds, based both on pre- and postmortem conditions, that may influence the quality of tissue and its ability to yield accurate results. The traditionally recognized confounds that reduce tissue quality are agonal factors (e.g., coma, hypoxia, hyperpyrexia at the time of death), and long postmortem interval (PMI). We measured tissue quality parameters in over 100 postmortem cases collected from different sources and correlated them with RNA quality (as indicated by the RNA Integrity Number (RIN)) and with protein quality (as measured by the level of representative proteins). Our results show that the most sensible indicator of tissue quality is RIN and that there is a good correlation between RIN and the pH. No correlation developed between protein levels and the aforementioned factors. Moreover, even when RNA was degraded, the protein levels remained stable. However, these correlations did not prove true under all circumstances (e.g., thawed tissue, surgical tissue), that yielded unexpected quality indicators. These data also suggest that cases whose source was a Medical Examiner's office represent high tissue quality.

AB - Postmortem human brain tissue is used for the study of many different brain diseases. A key factor in conducting postmortem research is the quality of the tissue. Unlike animal tissue, whose condition at death can be controlled and influenced, human tissue can only be collected naturalistically. This introduces potential confounds, based both on pre- and postmortem conditions, that may influence the quality of tissue and its ability to yield accurate results. The traditionally recognized confounds that reduce tissue quality are agonal factors (e.g., coma, hypoxia, hyperpyrexia at the time of death), and long postmortem interval (PMI). We measured tissue quality parameters in over 100 postmortem cases collected from different sources and correlated them with RNA quality (as indicated by the RNA Integrity Number (RIN)) and with protein quality (as measured by the level of representative proteins). Our results show that the most sensible indicator of tissue quality is RIN and that there is a good correlation between RIN and the pH. No correlation developed between protein levels and the aforementioned factors. Moreover, even when RNA was degraded, the protein levels remained stable. However, these correlations did not prove true under all circumstances (e.g., thawed tissue, surgical tissue), that yielded unexpected quality indicators. These data also suggest that cases whose source was a Medical Examiner's office represent high tissue quality.

KW - Brain

KW - pH

KW - PMI

KW - RIN

UR - http://www.scopus.com/inward/record.url?scp=33751181235&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751181235&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2006.09.025

DO - 10.1016/j.brainres.2006.09.025

M3 - Article

VL - 1123

SP - 1

EP - 11

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -